1. Solution structure of a two-base DNA bulge complexed with an enediyne cleaving analog
- Author
-
Stassinopoulos, Adonis, Ji, Jie, Gao, Xiaolian, and Goldberg, Irving H.
- Subjects
Nucleic acids -- Research ,Pharmaceutical microbiology -- Research ,Antibiotics -- Research ,Science and technology ,Research - Abstract
Nucleic acid bulges have been implicated in a number of biological processes and are specific cleavage targets for the enediyne antitumor antibiotic neocarzinostatin chromophore in a base-catalyzed, radical-mediated reaction. The solution structure of the complex between an analog of the bulge-specific cleaving species and an oligodeoxynucleotide containing a two-base bulge was elucidated by nuclear magnetic resonance. An unusual binding mode involves major groove recognition by the drug carbohydrate unit and tight fitting of the wedge-shaped drug in the triangular prism pocket formed by the two looped-out bulge bases and the neighboring base pairs. The two drug rings mimic helical DNA bases, complementing the bent DNA structure. The putative abstracting drug radical is 2.2 ± 0.1 angstroms from the pro-S H5' of the target bulge nucleotide. This structure clarifies the mechanism of bulge recognition and cleavage by a drug and provides insight into the design of bulge-specific nucleic acid binding molecules., Bulged structures (regions of unpaired bases) in nucleic acids have been the subject of intense interest (1), because they have been implicated as binding motifs for regulatory proteins in viral [...]
- Published
- 1996