1. Programmed adsorption and release of proteins in a microfluidic device.
- Author
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Huber DL, Manginell RP, Samara MA, Kim BI, and Bunker BC
- Subjects
- Acrylic Resins, Adsorption, Biochemistry instrumentation, Cytochrome c Group chemistry, Hemoglobins chemistry, Hydrophobic and Hydrophilic Interactions, Microchemistry, Miniaturization, Serum Albumin, Bovine chemistry, Spectrophotometry, Ultraviolet, Temperature, Biochemistry methods, Proteins chemistry
- Abstract
A microfluidic device has been developed that can adsorb proteins from solution, hold them with negligible denaturation, and release them on command. The active element in the device is a 4-nanometer-thick polymer film that can be thermally switched between an antifouling hydrophilic state and a protein-adsorbing state that is more hydrophobic. This active polymer has been integrated into a microfluidic hot plate that can be programmed to adsorb and desorb protein monolayers in less than 1 second. The rapid response characteristics of the device can be manipulated for proteomic functions, including preconcentration and separation of soluble proteins on an integrated fluidics chip.
- Published
- 2003
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