1. The Trypanosoma cruzi proteome.
- Author
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Atwood JA 3rd, Weatherly DB, Minning TA, Bundy B, Cavola C, Opperdoes FR, Orlando R, and Tarleton RL
- Subjects
- Adaptation, Physiological, Animals, Antigens, Protozoan analysis, Chromatography, Liquid, Computational Biology, Databases, Genetic, Energy Metabolism, Enzymes genetics, Enzymes metabolism, Genes, Protozoan, Genome, Protozoan, Glycoproteins analysis, Glycoproteins genetics, Histidine metabolism, Life Cycle Stages, Mass Spectrometry, Membrane Proteins analysis, Membrane Proteins genetics, Mucins analysis, Multigene Family, Neuraminidase analysis, Neuraminidase genetics, Peptides analysis, Protein Transport, Protozoan Proteins genetics, Protozoan Proteins metabolism, Trypanosoma cruzi genetics, Trypanosoma cruzi metabolism, Proteome, Protozoan Proteins analysis, Trypanosoma cruzi chemistry, Trypanosoma cruzi growth & development
- Abstract
To complement the sequencing of the three kinetoplastid genomes reported in this issue, we have undertaken a whole-organism, proteomic analysis of the four life-cycle stages of Trypanosoma cruzi. Peptides mapping to 2784 proteins in 1168 protein groups from the annotated T. cruzi genome were identified across the four life-cycle stages. Protein products were identified from >1000 genes annotated as "hypothetical" in the sequenced genome, including members of a newly defined gene family annotated as mucin-associated surface proteins. The four parasite stages appear to use distinct energy sources, including histidine for stages present in the insect vectors and fatty acids by intracellular amastigotes.
- Published
- 2005
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