1. Cilia at the Node of Mouse Embryos Sense Fluid Flow for Left-Right Determination via Pkd2.
- Author
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Yoshiba, Satoko, Shiratori, Hidetaka, Kuo, Ivana Y., Kawasumi, Aiko, Shinohara, Kyosuke, Nonaka, Shigenori, Asai, Yasuko, Sasaki, Genta, Belo, Jose Antonio, Sasaki, Hiroshi, Nakai, Junichi, Dworniczak, Bernd, Ehrlich, Barbara E., Pennekamp, Petra, and Hamada, Hiroshi
- Subjects
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SYMMETRY (Biology) , *EMBRYOLOGY , *CILIA & ciliary motion , *FLUID dynamics , *POLYCYSTINS , *CALCIUM channels , *CALCIUM in the body , *KIDNEY physiology , *GENE expression - Abstract
The article discusses left-right (L-R) symmetry breaking in mammal embryogenesis, with particular focus on the function of unidirectional ventral node fluid flow and its sensing by cell cilia. Researchers looked at the role of Polycystin-2 (Pkd2) calcium channel localization in perinodal crown cell cilia of kidneys, finding that mice embryos with a mutated Pkd2 gene unable to attach to cilia were unable to sense nodal flow. Other topics include the distinction between motile and immotile cilia, the asymmetric expression of the Pkd2 target protein Cerl2 and Nodal protein, and calcium signalling in crown cells.
- Published
- 2012
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