1. CKAMP44: a brain-specific protein attenuating short-term synaptic plasticity in the dentate gyrus
- Author
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von Engelhardt, Jakob, Mack, Volker, Sprengel, Rolf, Kavenstock, Netta, Li, Ka Wan, Stern-Bach, Yael, Smit, August B., Seeburg, Peter H., and Monyer, Hannah
- Subjects
Neuroplasticity -- Research ,Dentate gyrus -- Chemical properties ,Membrane proteins -- Properties ,Science and technology - Abstract
CKAMP44, identified here by a proteomic approach, is a brain-specific type I transmembrane protein that associates with AMPA receptors in synaptic spines. CKAMP44 expressed in Xenopus oocytes reduced GluA1-and A2-mediated steady-state currents, but did not affect kainate-or N-methyl-D-aspartate (NMDA) receptor--mediated currents. Mouse hippocampal CA1 pyramidal neurons expressed CKAMP44 at low abundance, and overexpression of CKAMP44 led to stronger and faster AMPA receptor desensitization, slower recovery from desensitization, and a reduction in the paired-pulse ratio of AMPA currents. By contrast, dentate gyrus granule cells exhibited strong CKAMP44 expression, and CKAMP44 knockout increased the paired-pulse ratio of AMPA currents in lateral and medial perforant path--granule ceil synapses. CKAMP44 thus modulates short-term plasticity at specific excitatory synapses. 10.1126/science.1184178
- Published
- 2010
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