1. Cytoskeletal Regulation by the Nedd8 Ubiquitin-Like Protein Modification Pathway
- Author
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John H. Willis, Matthias Peter, Bruce Bowerman, Thimo Kurz, Lionel Pintard, Pierre Gönczy, and Danielle R. Hamill
- Subjects
Cell Nucleus/physiology/ultrastructure ,Embryo, Nonmammalian ,Microtubules/drug effects/*physiology/ultrastructure ,Ubiquitins/genetics/*metabolism ,Cell Membrane/ultrastructure ,Cell Cycle Proteins ,P.H.S ,Microtubules ,NEDD8 ,Pronuclear migration ,Non-U.S. Gov't ,Genes, Helminth ,Adenosine Triphosphatases ,Multidisciplinary ,Nocodazole ,Helminth/genetics ,Cullin Proteins ,Nocodazole/pharmacology ,Cell biology ,Ubiquitin ligase ,Actin Cytoskeleton ,Meiosis ,Embryo ,Mitotic Spindle Apparatus/physiology/ultrastructure ,Katanin ,Caenorhabditis elegans/*embryology/genetics/metabolism ,Cell Division ,Cullin ,Caenorhabditis elegans Proteins/genetics/*metabolism ,Mitosis ,Spindle Apparatus ,Biology ,Research Support ,Nonmammalian/metabolism ,Helminth ,Animals ,Caenorhabditis elegans ,Caenorhabditis elegans Proteins ,Ubiquitins ,Cell Nucleus ,Cell Membrane ,Spindle apparatus ,Genes ,Adenosinetriphosphatase/genetics/*metabolism ,Mutation ,biology.protein ,Microfilaments/*physiology/ultrastructure ,RNA ,U.S. Gov't ,RNA, Helminth ,Cell Cycle Proteins/genetics/metabolism ,Cytokinesis - Abstract
The Nedd8 ubiquitin-like protein modification pathway regulates cell-cycle progression. Our analysis of Nedd8 requirements during Caenorhabditis elegans embryogenesis indicates that the cytoskeleton is another target. Nedd8 conjugation negatively regulated contractility of the microfilament-rich cell cortex during pronuclear migration and again during cytokinesis. The Nedd8 pathway also was required after meiosis to negatively regulate katanin, a microtubule-severing complex, permitting the assembly of a large mitotic spindle. We propose that Nedd8-modified cullin, as part of an E3 ubiquitin ligase complex, targets katanin for degradation during the transition from meiosis to mitosis.
- Published
- 2002
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