1. Transcription-Coupled Events Associating with Immunoglobulin Switch Region Chromatin
- Author
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Yukiko Nambu, Hiroyuki Gonda, Tomoya Katakai, Manabu Sugai, Yasutoshi Agata, Akira Shimizu, Chung-Gi Lee, and Yoshifumi Yokota
- Subjects
Lipopolysaccharides ,Transcription, Genetic ,chemical and pharmacologic phenomena ,Hydroxamic Acids ,Lymphocyte Activation ,Immunoglobulin Switch Region ,Histones ,Transforming Growth Factor beta1 ,Mice ,Transforming Growth Factor beta ,Transcription (biology) ,Cytidine Deaminase ,Activation-induced (cytidine) deaminase ,Animals ,Cells, Cultured ,Recombination, Genetic ,Genetics ,B-Lymphocytes ,Multidisciplinary ,biology ,Acetylation ,DNA ,Cytidine deaminase ,Immunoglobulin E ,Immunoglobulin Class Switching ,Precipitin Tests ,Chromatin ,Cell biology ,Histone Deacetylase Inhibitors ,Histone ,Immunoglobulin class switching ,Immunoglobulin G ,biology.protein ,RNA ,Interleukin-4 ,RNA Polymerase II - Abstract
Class switch recombination (CSR) at the antibody immunoglobulin locus is regulated by germline transcription (GLT)–coupled modifications in the accessibility of the switch region, where CSR takes place. Here we show that histone acetylation of switch regions is linked to CSR but that histone acetylation cannot alone promote CSR or GLT. Activation-induced cytidine deaminase (AID) specifically associates with the CSR target chromatin in a GLT-coupled manner, which may occur potentially by means of physical interaction between AID and the transcription machinery. These data indicate an important role of GLT in the regulation of chromatin accessibility, strongly suggesting that the target of AID is chromatin DNA. Our results give insights on the role of AID and the regulatory mechanism of CSR.
- Published
- 2003
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