1. Site-Specific Oligonucleotide Binding Represses Transcription of the Human c- myc Gene in Vitro
- Author
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S. J. Flint, E H Postel, M Cooney, G Czernuszewicz, and M E Hogan
- Subjects
Electrophoresis ,Multidisciplinary ,Transcription, Genetic ,Base pair ,Oligonucleotide ,Nucleic Acid Hybridization ,In Vitro Techniques ,Biology ,Molecular biology ,Nucleic acid thermodynamics ,chemistry.chemical_compound ,Gene Expression Regulation ,Oligodeoxyribonucleotides ,chemistry ,Regulatory sequence ,Transcription (biology) ,Proto-Oncogene Proteins ,Proto-Oncogenes ,Humans ,Binding site ,Gene ,DNA - Abstract
A 27-base-long DNA oligonucleotide was designed that binds to duplex DNA at a single site within the 5' end of the human c-myc gene, 115 base pairs upstream from the transcription origin P1. On the basis of the physical properties of its bound complex, it was concluded that the oligonucleotide forms a colinear triplex with the duplex binding site. By means of an in vitro assay system, it was possible to show a correlation between triplex formation at -115 base pairs and repression of c-myc transcription. The possibility is discussed that triplex formation (site-specific RNA binding to a DNA duplex) could serve as the basis for an alternative program of gene control in vivo.
- Published
- 1988
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