1. The Genome of the Kinetoplastid Parasite,Leishmania major
- Author
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Kathy Seeger, G. Bothe, Rob Squares, Lee Murphy, Valentina Tosato, Michael A. Quail, Angela Lord, Alasdair Ivens, Christopher S. Peacock, Simon Rutter, Joel Rinta, Audrey Fraser, Dhileep Sivam, Carlo V. Bruschi, Nigel Fosker, Arlette Goble, Jenefer M. Blackwell, Silke Müller-Auer, K. Borzym, Robert L. Davies, Heather Munden, Holger Wedler, André Goffeau, Ellen Adlem, Christy Vogt, Susan O'Neil, David C. Schwartz, Siri Nelson, Bénédicte Purnelle, Angela K. Cruz, Martin Pentony, Deborah F. Smith, Michael A. Rieger, Laura Ciarloni, Kenneth Stuart, Marco A. Marra, Sven Klages, David L. Saunders, Alfred Beck, Marie-Adèle Rajandream, David Masuy, Andrew J Knights, Atashi Anupama, Karen Oliver, Helmut Hilbert, Rolf Wambutt, David Harris, Gabriella Bianchettin, Sarah Sharp, Nathalie Bason, Alberto C.C. Frasch, Matt Collins, Heesun Shin, Jeronimo C. Ruiz, Jeremy C. Mottram, David Horn, Richard M.R. Coulson, Elizabeth A. Worthey, Amber Seyler, Christopher Bauser, Natasha Larke, Bart Barrell, Ester Rabbinowitsch, John Woodward, Zina Apostolou, Christine Clayton, Christiane Hertz-Fowler, Ellen Sisk, Steve Squares, Gholam Fazelina, Laura Robertson, Johan Robben, T. Warren, Halina Norbertczak, Javier G. De Gaudenzi, Monika Fuchs, Shulamit Michaeli, Matthew Berriman, Claudia Gabel, Melanie Schäfer, Peter J. Myler, Thomas M. Pohl, Tin Louie, Rita Aert, Claire Price, Stephen M. Beverley, Guido Volckaert, Deborah E. Dobson, Richard Reinhardt, Lyudmila Litvin, Andreas Duesterhoeft, Ann Cronin, Jacquie Schein, Shiguo Zhou, Wolfgang Zimmermann, Gautam Aggarwal, Eithon Cadag, Yiting Huang, Michael Kube, Philip Attipoe, and Keith R. Matthews
- Subjects
Transcription, Genetic ,RNA Splicing ,Pseudogene ,family encoding amastin ,Genes, Protozoan ,Molecular Sequence Data ,Protozoan Proteins ,Leishmaniasis, Cutaneous ,Biology ,Genome ,Article ,chemistry.chemical_compound ,Transcription (biology) ,RNA polymerase ,parasitic diseases ,Animals ,RNA Processing, Post-Transcriptional ,gene ,Gene ,Leishmania major ,Genetics ,Multidisciplinary ,General transcription factor ,Proteolytic enzymes ,Membrane Proteins ,RNA ,Genes, rRNA ,differentiation ,Sequence Analysis, DNA ,organization ,Lipid Metabolism ,pre-messenger-rna ,Chromatin ,Gene Expression Regulation ,chemistry ,Multigene Family ,Protein Biosynthesis ,friedlin chromosome-1 ,trypanosoma-brucei ,cruzi ,transcription ,protein ,Genome, Protozoan ,Glycoconjugates ,Protein Processing, Post-Translational ,RNA, Protozoan - Abstract
Leishmaniaspecies cause a spectrum of human diseases in tropical and subtropical regions of the world. We have sequenced the 36 chromosomes of the 32.8-megabase haploid genome ofLeishmania major(Friedlin strain) and predict 911 RNA genes, 39 pseudogenes, and 8272 protein-coding genes, of which 36% can be ascribed a putative function. These include genes involved in host-pathogen interactions, such as proteolytic enzymes, and extensive machinery for synthesis of complex surface glycoconjugates. The organization of protein-coding genes into long, strand-specific, polycistronic clusters and lack of general transcription factors in theL. major, Trypanosoma brucei, andTrypanosoma cruzi(Tritryp) genomes suggest that the mechanisms regulating RNA polymerase IIâdirected transcription are distinct from those operating in other eukaryotes, although the trypanosomatids appear capable of chromatin remodeling. Abundant RNA-binding proteins are encoded in the Tritryp genomes, consistent with active posttranscriptional regulation of gene expression.
- Published
- 2005
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