1. Insights into neutralizing antibody responses in individuals exposed to SARS-CoV-2 in Chile
- Author
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Eugenio Ramírez, Fernando Valiente-Echeverría, Nicole Le Corre, María Elvira Balcells, Sebastián Riquelme-Barrios, Constanza Martínez-Valdebenito, Aracelly Gaete-Argel, Aarón Oyarzún-Arrau, Dante Travisany, Ricardo Soto-Rifo, Roxana Gonzalez-Stegmaier, Adam Aguirre, Franz Villarroel, Jorge Fernández, Ricardo Palma-Vejares, Carolina Beltrán-Pavez, Gonzalo P. Barriga, Karina Cereceda-Solis, Marcela Ferrés, and Aldo Gaggero
- Subjects
Male ,Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) ,viruses ,Population ,Immunology ,Mutation, Missense ,Antibodies, Viral ,Neutralization ,COVID-19 Serological Testing ,03 medical and health sciences ,0302 clinical medicine ,Immunity ,Virology ,Chlorocebus aethiops ,Animals ,Humans ,030212 general & internal medicine ,Chile ,Neutralizing antibody ,education ,skin and connective tissue diseases ,Vero Cells ,Research Articles ,030304 developmental biology ,0303 health sciences ,education.field_of_study ,Multidisciplinary ,biology ,SARS-CoV-2 ,fungi ,Wild type ,virus diseases ,SciAdv r-articles ,COVID-19 ,Antibodies, Neutralizing ,body regions ,Coronavirus ,HEK293 Cells ,Amino Acid Substitution ,Spike Glycoprotein, Coronavirus ,biology.protein ,Vero cell ,Female ,Antibody ,Research Article - Abstract
An HIV-1–based pseudotype allows the rapid quantification of neutralizing antibody titers against the SARS-CoV-2 spike protein., Chile has one of the worst numbers worldwide in terms of SARS-CoV-2 positive cases and COVID-19–related deaths per million inhabitants; thus, characterization of neutralizing antibody (NAb) responses in the general population is critical to understanding of immunity at the local level. Given our inability to perform massive classical neutralization assays due to the scarce availability of BSL-3 facilities in the country, we developed and fully characterized an HIV-based SARS-CoV-2 pseudotype, which was used in a 96-well plate format to investigate NAb responses in samples from individuals exposed to SARS-CoV-2 or treated with convalescent plasma. We also identified samples with decreased or enhanced neutralization activity against the D614G spike variant compared with the wild type, indicating the relevance of this variant in host immunity. The data presented here represent the first insights into NAb responses in individuals from Chile, serving as a guide for future studies in the country.
- Published
- 2021
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