1. Genome Sequence of the PCE-Dechlorinating Bacterium Dehalococcoides ethenogenes
- Author
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Kevin Tran, Hoda Khouri, Robert T. DeBoy, Karen E. Nelson, Steven A. Sullivan, Stephen H. Zinder, Marjorie Impraim, William C. Nelson, Derrick E. Fouts, John F. Heidelberg, Ramana Madupu, James F. Kolonay, Adam M. Phillippy, Naomi L. Ward, Jonathan A. Eisen, Sean C. Daugherty, Heather Forberger, Robert J. Dodson, Claire M. Fraser, Lauren M. Brinkac, Rekha Seshadri, Lorenz Adrian, Barbara A. Methé, Jeffrey M. Robinson, and Katherine H. Kang
- Subjects
Tetrachloroethylene ,Hydrogenase ,General Science & Technology ,Molecular Sequence Data ,Chemical ,Dehalobacter ,Genome ,Environmental ,Gene Duplication ,Nitrogenase ,Operon ,Genetics ,Water Pollutants ,Amino Acids ,Gene ,Desulfitobacterium ,Dehalogenase ,Dehalococcoides ,Whole genome sequencing ,Multidisciplinary ,biology ,Human Genome ,Bacterial ,Quinones ,DNA ,Chloroflexi ,Sequence Analysis, DNA ,biology.organism_classification ,Biodegradation, Environmental ,Genes ,Genes, Bacterial ,Biodegradation ,Oxidoreductases ,Sequence Analysis ,Oxidation-Reduction ,Genome, Bacterial ,Water Pollutants, Chemical ,Biotechnology ,Hydrogen ,Transcription Factors - Abstract
Dehalococcoides ethenogenes is the only bacterium known to reductively dechlorinate the groundwater pollutants, tetrachloroethene (PCE) and trichloroethene, to ethene. Its 1,469,720âbase pair chromosome contains large dynamic duplicated regions and integrated elements. Genes encoding 17 putative reductive dehalogenases, nearly all of which were adjacent to genes for transcription regulators, and five hydrogenase complexes were identified. These findings, plus a limited repertoire of other metabolic modes, indicate that D. ethenogenes is highly evolved to utilize halogenated organic compounds and H 2 . Diversification of reductive dehalogenase functions appears to have been mediated by recent genetic exchange and amplification. Genome analysis provides insights into the organism's complex nutrient requirements and suggests that an ancestor was a nitrogen-fixing autotroph.
- Published
- 2005
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