1. Calorie Restriction Promotes Mitochondrial Biogenesis by Inducing the Expression of eNOS
- Author
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Michele O. Carruba, Orazio Cantoni, Renata Bracale, Emilio Clementi, Enzo Nisoli, A Cardile, Cristina Tonello, Laura Tedesco, Alessandra Valerio, Valeria Cozzi, Sestina Falcone, and Salvador Moncada
- Subjects
Male ,Nitric Oxide Synthase Type III ,Calorie restriction ,Nitric Oxide Synthase Type II ,Biology ,Mitochondrion ,Nitric Oxide ,DNA, Mitochondrial ,GTP Phosphohydrolases ,Nitric oxide ,Mice ,chemistry.chemical_compound ,Life Expectancy ,Oxygen Consumption ,Sirtuin 1 ,Enos ,CALORIE RESTRICTION ,Animals ,Sirtuins ,Caloric Restriction ,Multidisciplinary ,MITOCHONDRIAL BIOGENESIS ,biology.organism_classification ,Mitochondria ,Cell biology ,Mice, Inbred C57BL ,eNOS ,Nitric oxide synthase ,Adipose Tissue ,Biochemistry ,Mitochondrial biogenesis ,chemistry ,Enzyme Induction ,Protein Biosynthesis ,biology.protein ,Female ,Nitric Oxide Synthase - Abstract
Calorie restriction extends life span in organisms ranging from yeast to mammals. Here, we report that calorie restriction for either 3 or 12 months induced endothelial nitric oxide synthase (eNOS) expression and 3′,5′-cyclic guanosine monophosphate formation in various tissues of male mice. This was accompanied by mitochondrial biogenesis, with increased oxygen consumption and adenosine triphosphate production, and an enhanced expression of sirtuin 1. These effects were strongly attenuated in eNOS null-mutant mice. Thus, nitric oxide plays a fundamental role in the processes induced by calorie restriction and may be involved in the extension of life span in mammals.
- Published
- 2005
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