1. Signaling and Circuitry of Multiple MAPK Pathways Revealed by a Matrix of Global Gene Expression Profiles
- Author
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Matthew J. Marton, Stephen H. Friend, Wynn L. Walker, Roland Stoughton, Timothy P. Hughes, Yudong D. He, Charles Boone, Bryce Nelson, Mike Tyers, Michael R. Meyer, Holly A. Bennett, Hongyue Dai, and Christopher J. Roberts
- Subjects
Transcriptional Activation ,Saccharomyces cerevisiae Proteins ,MAP Kinase Signaling System ,Lipoproteins ,Cell Cycle Proteins ,Saccharomyces cerevisiae ,Biology ,Pheromones ,Fungal Proteins ,Gene Expression Regulation, Fungal ,Gene expression ,Gene ,Transcription factor ,Protein Kinase C ,Ste5 ,Cyclin-Dependent Kinase Inhibitor Proteins ,Oligonucleotide Array Sequence Analysis ,Genetics ,Fungal protein ,Multidisciplinary ,Gene Expression Profiling ,G1 Phase ,Cell biology ,Repressor Proteins ,Gene expression profiling ,Multigene Family ,Fus3 ,Genome, Fungal ,Mitogen-Activated Protein Kinases ,Signal transduction ,Mating Factor ,Peptides ,Transcription Factors - Abstract
Genome-wide transcript profiling was used to monitor signal transduction during yeast pheromone response. Genetic manipulations allowed analysis of changes in gene expression underlying pheromone signaling, cell cycle control, and polarized morphogenesis. A two-dimensional hierarchical clustered matrix, covering 383 of the most highly regulated genes, was constructed from 46 diverse experimental conditions. Diagnostic subsets of coexpressed genes reflected signaling activity, cross talk, and overlap of multiple mitogen-activated protein kinase (MAPK) pathways. Analysis of the profiles specified by two different MAPKs—Fus3p and Kss1p—revealed functional overlap of the filamentous growth and mating responses. Global transcript analysis reflects biological responses associated with the activation and perturbation of signal transduction pathways.
- Published
- 2000
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