1. Frequency and characterization of RHD variants in serologically D- Surinamese pregnant women and D- newborns.
- Author
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Zonneveld R, Kanhai HHH, Javadi A, Veldhuisen B, Brand A, Zijlmans WCWR, van der Schoot CE, and Schonewille H
- Subjects
- Adult, Cross-Sectional Studies, Erythroblastosis, Fetal blood, Erythroblastosis, Fetal genetics, Female, Humans, Infant, Newborn, Ligase Chain Reaction, Pregnancy, Real-Time Polymerase Chain Reaction, Rh-Hr Blood-Group System blood, Risk Factors, Suriname, Exons, Genetic Variation, Rh-Hr Blood-Group System genetics
- Abstract
Background: Numerous RHD variant genes affect the expression of D on the red blood cell surface. In Suriname, 4.3% of pregnant women were D-, ranging from virtually zero to 7% among ethnic groups. Characterization of RHD variants, which are associated with a variable potential to induce anti-D, is of practical clinical importance especially in case of limited access to preventive measures. Here we report on the occurrence of RHD variant genes in Surinamese serologically D- pregnant women and their D- newborns from different ethnic groups., Study Design and Methods: The RheSuN study is a cross-sectional cohort study in D- pregnant women and their newborns, who visited hospitals in Paramaribo, Suriname, during routine pregnancy care. The presence of RHD variants was investigated using quantitative polymerase chain reaction targeting RHD Exons 5 and 7 and RH-multiplex ligation-dependent probe amplification., Results: Seven RHD variant genes were detected in 35 of 84 women and four RHD variant genes in 15 of 36 newborns. The RHD*03 N.01 and RHD*08 N.01 variants represented 87% of a total of 62 variant genes. Variants were comparably frequent among ethnicities. In four cases genotyping would have changed anti-D prophylaxis policy: one woman with a RHD*01EL.01 variant, not associated with anti-D formation and three D- newborns with RHD*09.01 and RHD*09.03.01 variants, potentially capable of inducing anti-D., Conclusion: RHD variants at risk for anti-D are common among serologic D- individuals from African descent in Suriname. While genotyping D- women has limited added value, it may be considered in newborns from D- women., (© 2019 AABB.)
- Published
- 2019
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