1. Increased susceptibility to apoptosis of CD56dimCD16+ NK cells induces the enrichment of IFN-gamma-producing CD56bright cells in tuberculous pleurisy.
- Author
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Schierloh P, Yokobori N, Alemán M, Musella RM, Beigier-Bompadre M, Saab MA, Alves L, Abbate E, de la Barrera SS, and Sasiain MC
- Subjects
- Adult, Aged, Antigens, CD metabolism, Antigens, CD19 metabolism, CD3 Complex metabolism, CD56 Antigen metabolism, GPI-Linked Proteins, Humans, In Vitro Techniques, Interferon-gamma biosynthesis, Killer Cells, Natural classification, Lipopolysaccharide Receptors metabolism, Lymphocyte Subsets immunology, Lymphocyte Subsets pathology, Middle Aged, Phenotype, Pleural Effusion immunology, Pleural Effusion pathology, Receptors, IgG metabolism, Apoptosis immunology, Killer Cells, Natural immunology, Killer Cells, Natural pathology, Tuberculosis, Pleural immunology, Tuberculosis, Pleural pathology
- Abstract
Tuberculous pleuritis is a good model for the study of specific cells at the site of active Mycobacterium tuberculosis (Mtb) infection. We investigated the frequency and phenotype of NK cells in paired samples of peripheral blood and pleural fluid (PF) from patients with tuberculosis (TB) or parapneumonic infection. We demonstrated for the first time a reduction of NK cells in PF from TB with an enrichment in the CD56brightCD16- subset. In agreement, in PF NK cells we observed an increased expression of CD94, NKG2A, CD62L, and CCR7 molecules and lower expression of Bcl-2 and perforin. The activation markers CD69 and HLA-DR were also increased. The enrichment in the CD56bright subset was due to an increased susceptibility to apoptosis of CD56+CD16+ NK cells mediated by heat-labile and stable soluble factors present in tuberculous effusions and not in PF from other etiologies. Furthermore, in TB patients, Mtb-induced IFN-gamma production by PF NK cells was not dependent on the presence of CD3+, CD19+, and CD14+ cells, suggesting a direct interaction of CD56bright cells with Mtb and/or the involvement of other accessory cells present at the site of Mtb infection.
- Published
- 2005
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