1. Effects of Plant Sterols or β-Cryptoxanthin at Physiological Serum Concentrations on Suicidal Erythrocyte Death.
- Author
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Alvarez-Sala A, López-García G, Attanzio A, Tesoriere L, Cilla A, Barberá R, and Alegría A
- Subjects
- Beta-Cryptoxanthin blood, Cells, Cultured, Cholesterol analogs & derivatives, Cholesterol pharmacology, Erythrocytes chemistry, Erythrocytes drug effects, Glutathione blood, Hemolysis drug effects, Humans, Oxidative Stress drug effects, Sitosterols pharmacology, Stigmasterol pharmacology, tert-Butylhydroperoxide pharmacology, Beta-Cryptoxanthin pharmacology, Eryptosis drug effects, Phytosterols pharmacology
- Abstract
The eryptotic and hemolytic effects of a phytosterol (PS) mixture (β-sitosterol, campesterol, stigmasterol) or β-cryptoxanthin (β-Cx) at physiological serum concentration and their effect against oxidative stress induced by tert-butylhydroperoxide (tBOOH) (75 and 300 μM) were evaluated. β-Cryptoxanthin produced an increase in eryptotic cells, cell volume, hemolysis, and glutathione depletion (GSH) without ROS overproduction and intracellular Ca
2+ influx. Co-incubation of both bioactive compounds protected against β-Cx-induced eryptosis. Under tBOOH stress, PS prevented eryptosis, reducing Ca2+ influx, ROS overproduction and GSH depletion at 75 μM, and hemolysis at both tBOOH concentrations. β-Cryptoxanthin showed no cytoprotective effect. Co-incubation with both bioactive compounds completely prevented hemolysis and partially prevented eryptosis as well as GSH depletion induced by β-Cx plus tBOOH. Phytosterols at physiological serum concentrations help to prevent pro-eryptotic and hemolytic effects and are promising candidate compounds for ameliorating eryptosis-associated diseases.- Published
- 2018
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