1. The single-probe: a miniaturized multifunctional device for single cell mass spectrometry analysis.
- Author
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Pan N, Rao W, Kothapalli NR, Liu R, Burgett AW, and Yang Z
- Subjects
- Adenosine Diphosphate analysis, Adenosine Diphosphate metabolism, Adenosine Monophosphate analysis, Adenosine Monophosphate metabolism, Adenosine Triphosphate analysis, Adenosine Triphosphate metabolism, Cholestenones analysis, Doxorubicin analysis, HeLa Cells, Humans, Mass Spectrometry methods, Paclitaxel analysis, Phosphatidylcholines analysis, Phosphatidylcholines metabolism, Saponins analysis, Single-Cell Analysis methods, Antineoplastic Agents analysis, Mass Spectrometry instrumentation, Metabolome, Single-Cell Analysis instrumentation
- Abstract
We have developed a new mass spectrometry (MS) technology, the Single-probe MS, capable of real-time, in situ metabolomic analysis of individual living cells. The Single-probe is a miniaturized multifunctional sampling and ionization device that is directly coupled to the mass spectrometer. With a sampling tip smaller than individual eukaryotic cells (<10 μm), the Single-probe can be inserted into single cells to sample the intracellular compounds for real-time MS analysis. We have used the Single-probe to detect several cellular metabolites and the anticancer small molecules paclitaxel, doxorubicin, and OSW-1 in individual cervical cancer cells (HeLa). Single cell mass spectrometry (SCMS) is an emerging scientific technology that could reshape the analytical science of many research disciplines, and the Single-probe MS technology is a novel method for SCMS that, through its accessible fabrication protocols, can be broadly applied to different research areas.
- Published
- 2014
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