1. Boronate probes as diagnostic tools for real time monitoring of peroxynitrite and hydroperoxides.
- Author
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Zielonka J, Sikora A, Hardy M, Joseph J, Dranka BP, and Kalyanaraman B
- Subjects
- Animals, Cell Line, Chromatography, High Pressure Liquid, Hydrogen Peroxide toxicity, Hypochlorous Acid analysis, Kinetics, Macrophages drug effects, Mice, Oxidation-Reduction, Peroxynitrous Acid toxicity, Boronic Acids chemistry, Fluorescent Dyes chemistry, Hydrogen Peroxide analysis, Mass Spectrometry, Peroxynitrous Acid analysis
- Abstract
Boronates, a group of organic compounds, are emerging as one of the most effective probes for detecting and quantifying peroxynitrite, hypochlorous acid, and hydrogen peroxide. Boronates react with peroxynitrite nearly a million times faster than with hydrogen peroxide. Boronate-containing fluorogenic compounds have been used to monitor real time generation of peroxynitrite in cells and for imaging hydrogen peroxide in living animals. This perspective highlights potential applications of boronates and other fluorescent probes to high-throughput analyses of peroxynitrite and hydroperoxides in toxicological studies.
- Published
- 2012
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