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Your search keyword '"Gill, Timothy"' showing total 2 results
Start Over Author "Gill, Timothy" Publisher american chemical society
2 results on '"Gill, Timothy"'

1. Substrate specificity of Fpg (MutM) and hoGG1, two repair glycosylases

Author

Hamm, Michelle L., Gill, Timothy J., Nicolson, Sarah C., and Summers, Matthew R.

Subjects
Restriction enzymes, DNA -- Structure, Restriction enzymes, DNA -- Research, High performance liquid chromatography -- Analysis, Thermodynamics -- Analysis, Chemistry
Abstract

The analysis investigates the structure and substrate specificity of Fpg (MutM) and hoGG1, the two enzymes that make up the glycosylases (GO) repair system. The results prove the existence of broad substrate specificity at the imidazole ring of 8-oxo-2'-deoxyguanosine (OdG).

Published
2007

2. Oligonucleotide incorporation of 8-thio-2'-deoxyguanosine.

Author

Hamm ML, Cholera R, Hoey CL, and Gill TJ

Subjects
Base Sequence, Chromatography, High Pressure Liquid, DNA chemical synthesis, Deoxyguanosine chemistry, Oxidation-Reduction, Deoxyguanosine analogs & derivatives, Oligonucleotides chemistry
Abstract

[reaction: see text] 8-Thio-2'-deoxyguanosine (SdG) is a useful analogue of the abundant promutagen 8-oxo-2'-deoxyguanosine (OdG). Its synthesis and DNA incorporation using standard phosphoramidite chemistry is reported. To prevent oxidation during DNA synthesis, the sulfur was protected as a 2-(trimethylsilyl)ethyl sulfide. Subsequent treatment with TBAF yielded the desired 8-thiocarbonyl functionality. Melting studies with SdG revealed almost equal stabilities of SdG:dC and SdG:dA base pairs, lending insight into the base-pairing preferences of OdG.

Published
2004
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