1. Single-cell measurements of human neutrophil activation using electrorotation.
- Author
-
Griffith AW and Cooper JM
- Subjects
- Cell Adhesion drug effects, Chemotaxis, Leukocyte drug effects, Drug Evaluation, Preclinical methods, Electrochemistry methods, Electromagnetic Fields, Humans, Neutrophils drug effects, Neutrophils physiology, Neutrophil Activation, Tetradecanoylphorbol Acetate
- Abstract
We have used the technique of cell electrorotation within a microfabricated planar gold electrode array as a means of detecting changes in the physical properties of a single human neutrophil, when activated by the chemotactic factor, phorbol myristate acetate (PMA). The results, which have been analyzed using a double-shell model to represent the cell and its nucleus, provide an indication of the changes in biophysical parameters that occur during cell activation. The methods used in this study have potential applications in the development of single-cell assays for pharmaceutical screening, as a means of determining rapidly the action of drugs.
- Published
- 1998
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