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A highly sensitive method to analyze the intact lipids in a single zooplankter individual at the level of a few tenths of a microgram was developed by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) combined with a direct sampling technique. The sampling procedure involved (1) putting a zooplankter individual sample onto the MALDI sample plate, (2) cutting the sample into a few pieces by means of tweezers, (3) depositing aliquots of matrix and cationization reagent solutions on the zooplankter sample, and (4) irradiating with a N[SUB2] laser to cause MALDI. By using this technique, the mass spectra of the single zooplankter samples showed a series of ions generated from phospholipids with 34 or 36 carbons in the acyl groups and neutral lipids such as triglycerides and diacylglyceryl ethers with 5054 carbons in their acyl and alkenyl groups. Accordingly, this method enabled us to estimate the relative quantity between "structured lipids" (phospholipids) and "storage lipids" (neutral lipids) in an individual zooplankter, which should give us a good clue to elucidate the roles of each class of lipids in its growth. [ABSTRACT FROM AUTHOR]

Discusses the characterization of natural resin shellac by reactive pyrolysis-gas chromatography in the presence of organic alkali, tetramethylammonium hydroxide. Determination of terpenic acids, aleuritic acid, several minor fatty acids and wax components of shellac; Distribution of acidic components for shellac samples produced in India and Thailand.

Determines styrene copolymer sizing agents in paper by pyrolysis gas chromatography. Use of styrene monomer derived from the styrene sequences; Determination of the amounts of sizing agents retained in paper samples; Interpretation of the amounts of sizing agents in paper by degree of sizing.

Presents a quantitative analysis of detailed lignin monomer composition by pyrolysis-gas chromatography. Contribution of the lignin acetylation procedure; Determination of the dehydrogenative polymers; Accuracy of the preacetylation in analyzing the detailed lignin monomer composition.