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Your search keyword '"Linning, P."' showing total 11 results
Start Over Author "Linning, P." Publisher american chemical society
11 results on '"Linning, P."'

9. A Rational Chemical Intervention Strategy To Circumvent Bioactivation Liabilities Associated with a Nonpeptidyl Thrombopoietin Receptor Agonist Containing a 2-Amino-4-arylthiazole Motif.

Author

Amit S. Kalgutkar, James Driscoll, Sabrina X. Zhao, Gregory S. Walker, Richard M. Shepard, John R. Soglia, James Atherton, Linning Yu, Abdul E. Mutlib, Michael J. Munchhof, Lawrence A. Reiter, Christopher S. Jones, Johnathan L. Doty, Kristen A. Trevena, Christopher L. Shaffer, and Sharon L. Ripp

Published
2007
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11. Analysis of amino-terminal variants of amyloid-β peptides by capillary isoelectric focusing immunoassay.

Author

Haußmann U, Jahn O, Linning P, Janßen C, Liepold T, Portelius E, Zetterberg H, Bauer C, Schuchhardt J, Knölker HJ, Klafki H, and Wiltfang J

Subjects
Amino Acid Sequence, Amyloid beta-Peptides genetics, Capillary Action, Cell Line, Tumor, Humans, Immunoassay methods, Molecular Sequence Data, Amyloid beta-Peptides analysis, Genetic Variation genetics, Isoelectric Focusing methods
Abstract

Here we present a novel assay for the separation and detection of amino-terminal amyloid-β (Aβ) peptide variants by capillary isoelectric focusing (CIEF) immunoassay. Specific amino-terminally truncated Aβ peptides appear to be generated by β-secretase (BACE1)-independent mechanisms and have previously been observed in cerebrospinal fluid (CSF) after BACE1 inhibitor treatment in an animal model. CIEF immunoassay sensitivity is sufficient to detect total Aβ in CSF without preconcentration. To analyze low-abundance amino-terminally truncated Aβ peptides from cell culture supernatants, we developed a CIEF-compatible immunoprecipitation protocol, allowing for selective elution of Aβ peptides with very low background. CIEF immunoassay and immunoprecipitation mass spectrometry analysis identified peptides starting at residue Arg(5) as the main amino-terminal Aβ variants produced in the presence of tripartite BACE1 inhibitor in our cell culture model. The CIEF immunoassay allows for robust relative quantification of Aβ peptide patterns in biological samples. To assess the future possibility of absolute quantification, we have prepared the Aβ peptides Aβ(x-10), Aβ(x-16), and Aβ(5-38(D23S)) by using solid phase peptide synthesis as internal standards for the CIEF immunoassay.

Published
2013
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