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Your search keyword '"Monnet, Véronique"' showing total 3 results
Start Over Author "Monnet, Véronique" Publisher american chemical society
3 results on '"Monnet, Véronique"'

3. Characterization of the insoluble proteome of Lactococcus lactis by SDS-PAGE LC-MS/MS leads to the identification of new markers of adaptation of the bacteria to the mouse digestive tract.

Author

Beganović J, Guillot A, van de Guchte M, Jouan A, Gitton C, Loux V, Roy K, Huet S, Monod H, and Monnet V

Subjects
Animals, Electrophoresis, Polyacrylamide Gel, Mice, Solubility, Adaptation, Physiological, Bacterial Proteins metabolism, Chromatography, Liquid methods, Lactococcus lactis metabolism, Proteome, Tandem Mass Spectrometry methods
Abstract

We characterized the insoluble proteome of Lactococcus lactis using 1D electrophoresis-LC-MS/MS and identified 313 proteins with at least two different peptides. The identified proteins include 89 proteins having a predicted signal peptide and 25 predicted to be membrane-located. In addition, 67 proteins had alkaline isoelectric point values. Using spectra and peptide counts, we compared protein abundances in two different conditions: growth in rich medium, and after transit in the mouse digestive tract. We identified the large mechanosensitive channel and a putative cation transporter as membrane markers of bacterial adaptation to the digestive tract.

Published
2010
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