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Your search keyword '"Rosenbergová, Šárka"' showing total 2 results
Start Over Author "Rosenbergová, Šárka" Publisher american chemical society
2 results on '"Rosenbergová, Šárka"'

2. Straightforward Synthesis of Purine 4'-Alkoxy-2'-deoxynucleosides: First Report of Mixed Purine-Pyrimidine 4'-Alkoxyoligodeoxynucleotides as New RNA Mimics.

Author

Petrová M, Páv O, Buděšínský M, Zborníková E, Novák P, Rosenbergová Š, Pačes O, Liboska R, Dvořáková I, Šimák O, and Rosenberg I

Subjects
Molecular Conformation, Molecular Structure, Nucleic Acid Conformation, Nucleic Acid Hybridization, Succinimides chemistry, Oligodeoxyribonucleotides chemical synthesis, Oligodeoxyribonucleotides chemistry, Oligonucleotides, Antisense chemistry, Purines chemistry, RNA chemistry
Abstract

Purine and pyrimidine 4'-alkoxy-2'-deoxynucleosides were efficiently prepared from nucleoside 4'-5'-enol acetates in three steps by N-iodosuccinimide promoted alkoxylation, hydrolysis, and reduction followed by conversion to phosphoramidite monomers for the solid-phase synthesis of the oligonucleotides. Fully modified 4'-alkoxyoligodeoxynucleotides, which are characterized by a prevalent N-type (RNA-like) conformation, exhibited superior chemical and nuclease resistance as well as excellent hybridization properties with a strong tendency for RNA-selective hybridization, suggesting a potential application of 4'-alkoxy-oligodeoxynucleotides in antisense technologies.

Published
2015
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