1. Delineation of the Olive Pollen Proteome and Its Allergenome Unmasks Cyclophilin as a Relevant Cross-Reactive Allergen.
- Author
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San Segundo-Acosta P, Oeo-Santos C, Benedé S, de Los Ríos V, Navas A, Ruiz-Leon B, Moreno C, Pastor-Vargas C, Jurado A, Villalba M, and Barderas R
- Subjects
- Allergens immunology, Allergens isolation & purification, Amino Acid Sequence genetics, Animals, Child, Chromatography, Liquid, Cross Reactions, Humans, Immunoglobulin E genetics, Immunoglobulin E immunology, Olea adverse effects, Olea genetics, Olea immunology, Pollen adverse effects, Pollen genetics, Pollen immunology, Proteome immunology, Proteomics, Tandem Mass Spectrometry, Allergens genetics, Antigens, Plant genetics, Cyclophilins genetics, Cyclophilins immunology, Proteome genetics
- Abstract
Olive pollen is a major allergenic source worldwide due to its extensive cultivation. We have combined available genomics data with a comprehensive proteomics approach to get the annotated olive tree ( Olea europaea L.) pollen proteome and define its complex allergenome. A total of 1907 proteins were identified by LC-MS/MS using predicted protein sequences from its genome. Most proteins (60%) were predicted to possess catalytic activity and be involved in metabolic processes. In total, 203 proteins belonging to 47 allergen families were found in olive pollen. A peptidyl-prolyl cis-trans isomerase, cyclophilin, produced in Escherichia coli , was found as a new olive pollen allergen (Ole e 15). Most Ole e 15-sensitized patients were children (63%) and showed strong IgE recognition to the allergen. Ole e 15 shared high sequence identity with other plant, animal, and fungal cyclophilins and presented high IgE cross-reactivity with pollen, plant food, and animal extracts.
- Published
- 2019
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