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Start Over Author "Suxia Zhang" Publisher american chemical society
11 results on '"Suxia Zhang"'

11. Development of a Monoclonal Antibody-Based Broad-Specificity ELISA for Fluoroquinolone Antibiotics in Foods and Molecular Modeling Studies of Cross-Reactive Compounds.

Author

Zhanhui Wang, Yan Zhu, Shuangyang Ding, Fangyang He, Beier, Ross C., Jiancheng Li, Haiyang Jiang, Caiwei Feng, Yuping Wan, Suxia Zhang, Zhenpeng Kai, Xinling Yang, and Jianzhong Shen

Subjects
*ENZYME-linked immunosorbent assay, *MONOCLONAL antibodies, *ANTIBIOTICS, *CIPROFLOXACIN, *NORFLOXACIN, *SERUM albumin, *ANTIGENS, *QUINOLONE antibacterial agents, *CHEMICAL reactions
Abstract

A competitive indirect enzyme-linked immunosorbent assay (ciELISA) using monoclonal antibodies (Mabs) having broad specificity for fluoroquinolone (FQ) antibiotics is described. Four FQs, ciprofloxacin (CIP), enrofloxacin (ENR), norfloxacin (NOR), and ofloxacin (OFL), were conjugated to bovine serum albumin for immunogens and to ovalbumin for coating antigens. A Mab C4A9H1 raised against the CIP hapten exhibited high cross-reactivity (35-100%) with 12 of 14 FQs and detected these FQs in a ciELISA below their maximum residue levels (MRLs) with good sensitivity at 50% binding inhibition (IC50). The quantitative structure-activity relationship (QSAR) between Mab C4A9H1 and various FQs by comparative molecular field analysis (CoMFA) showed a high predictive ability with a cross-validation q² value of 0.866. Using a simple purification process and the broad-specificity ciELISA adapted for analysis of FQs in chicken muscle, chicken liver, honey, shrimp, and whole egg samples demonstrated recoveries of 60–93% for CIP, ENR, NOR, OFL, flumequine, and danofloxacin. [ABSTRACT FROM AUTHOR]

Published
2007
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