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1. Solid-phase peptide synthesis in the reverse (N --> C) direction.

Author

Thieriet N, Guibé F, and Albericio F

Subjects
Biochemistry methods, Peptides chemical synthesis
Abstract

[reaction: see text] A new strategy for SPPS in the reverse direction based on the use of 2-Cl-trityl resin, an allyl ester as the temporary protecting group, and Cu(OBt)(2)/DIPCDI or HATU/DIEA as the coupling method is described. These conditions ensure good yields with minimal racemization of the C-terminal residue.

Published
2000
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