1. Gaussia luciferase variant for high-throughput functional screening applications.
- Author
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Maguire CA, Deliolanis NC, Pike L, Niers JM, Tjon-Kon-Fat LA, Sena-Esteves M, and Tannous BA
- Subjects
- Amino Acid Sequence, Animals, Catalysis, Luciferases chemistry, Molecular Sequence Data, Polymerase Chain Reaction, Sequence Homology, Amino Acid, Copepoda enzymology, Luciferases metabolism
- Abstract
Gaussia luciferase (Gluc) is a sensitive reporter for studying different biological processes such as gene expression, promoter activity, protein-protein interactions, signal transduction, as well as tumor cell growth and response to therapy. Since Gluc is naturally secreted, the kinetics of these processes can be monitored in real-time by measuring an aliquot of conditioned medium in culture or a few microliters of blood in vivo at different time points. Gluc catalyzes light emission with a short half-life which is unfavorable for certain applications. We isolated a Gluc mutant that catalyzes enhanced light stability in the presence of a detergent, in combination with high sensitivity, making it an attractive luciferase for high-throughput functional screening applications.
- Published
- 2009
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