13 results on '"Yang, Zhong-Yi"'
Search Results
2. Proteomic and Biochemical Evidence Involving Root Cell Wall Biosynthesis and Modification, Tricarboxylic Acid Cycle, and Glutathione Metabolism in Cultivar-Dependent Cd Accumulation of Water Spinach (Ipomoea aquatica).
- Author
-
Huang, Ying-Ying, Shen, Chuang, Fu, Hui-Ling, Xin, Jun-Liang, He, Chun-Tao, and Yang, Zhong-Yi
- Published
- 2023
- Full Text
- View/download PDF
3. Legacy and Currently Used Organic Contaminants in Human Hair and Hand Wipes of Female E‑Waste Dismantling Workers and Workplace Dust in South China.
- Author
-
Qiao, Lin, Zheng, Xiao-Bo, Zheng, Jing, Chen, She-Jun, Zhong, Chang-Qin, Chen, Jing-Hua, Yang, Zhong-Yi, and Mai, Bi-Xian
- Published
- 2019
- Full Text
- View/download PDF
4. Comparative Transcriptome Analysis Revealed the Tissue-Specific Accumulations of Taxanes among Three Experimental Lines of Taxus yunnanensis.
- Author
-
Mubeen S, Li ZL, Huang QM, He CT, and Yang ZY
- Subjects
- Biosynthetic Pathways, Bridged-Ring Compounds analysis, Gene Expression Profiling, Gene Expression Regulation, Plant, Plant Proteins metabolism, Taxoids analysis, Taxus chemistry, Taxus classification, Taxus metabolism, Bridged-Ring Compounds metabolism, Plant Proteins genetics, Taxoids metabolism, Taxus genetics
- Abstract
Taxus yunnanensis (Yew) is known for natural anticancer metabolite paclitaxel (Taxol) and its biosynthesis pathway in yew species still needs to be completely elucidated. In the current study, productions of paclitaxel and 10-DAB III from three different tissues (needle, branch, and root) of T. yunnanensis wild type (WT) and two new cultivars Zhongda-1 (Zd1) and Zhongda-2 (Zd2) were determined, and significant tissue differences in contents of the taxanes were observed among the three experimental lines. The much higher 10-DAB III and lower paclitaxel contents in needle of Zd2 when compared with that of Zd1 indicated the low conversion from 10-DAB III to paclitaxel in the needle of Zd2. In order to uncover the mechanisms of the tissue-specific biosynthesis of the taxanes, transcriptome analysis of cultivar Zd2 was conducted, and the previously reported transcriptome data of Zd1 and WT were used to perform a comparison. The enhancement of TDAT and T10βH side biosynthetic pathway in roots of Zd2 in early taxane synthesis might lead to the biosynthesis of other toxoids, while the preference of T13αH route in the needle and branch of Zd2 was mainly responsible for the tissue-specific reinforced biosynthesis of 10-DAB III and paclitaxel in Zd2. Different from Zd1, the tissue-specific pattern of paclitaxel biosynthesis genes in Zd2 was similar to WT. However, the lower transcript abundance of final steps genes (TBT, DBAT, BAPT, and DBTNBT) of the paclitaxel biosynthesis pathway in Zd2 than in Zd1 might further promote 10-DAB III accumulation in Zd2.
- Published
- 2018
- Full Text
- View/download PDF
5. Selection for Cd Pollution-Safe Cultivars of Chinese Kale (Brassica alboglabra L. H. Bailey) and Biochemical Mechanisms of the Cultivar-Dependent Cd Accumulation Involving in Cd Subcellular Distribution.
- Author
-
Guo JJ, Tan X, Fu HL, Chen JX, Lin XX, Ma Y, and Yang ZY
- Subjects
- Brassica growth & development, Brassica metabolism, Cadmium analysis, China, Consumer Product Safety, Humans, Plant Roots chemistry, Plant Roots growth & development, Plant Roots metabolism, Plant Shoots chemistry, Plant Shoots growth & development, Plant Shoots metabolism, Soil chemistry, Soil Pollutants analysis, Brassica chemistry, Cadmium metabolism, Soil Pollutants metabolism
- Abstract
Two pot experiments were conducted to compare and verify Cd accumulation capacities of different cultivars under Cd exposures (0.215, 0.543, and 0.925 mg kg
-1 in Exp-1 and 0.143, 0.619, and 1.407 mg kg-1 in Exp-2) and Cd subcellular distributions between low- and high-Cd cultivars. Shoot Cd concentrations between the selected low- and high-Cd cultivars were 1.4-fold different and the results were reproducible. The proportions of Cd-in-cell-wall of shoots and roots were all higher in a typical low-Cd cultivar (DX102) than in a typical high-Cd cultivar (HJK), while those of Cd-in-chloroplast or Cd-in-trophoplast and Cd-in-membrane-and-organelle were opposite. The proportions of Cd-in-vacuoles-and-cytoplasm of roots in DX102 were always higher than in HJK under Cd stresses, while there was no clear pattern in those of shoots. These findings may help to reduce health risk of Cd from Chinese kale consumption and explained biochemical mechanisms of cultivar-dependent Cd accumulation among the species.- Published
- 2018
- Full Text
- View/download PDF
6. Different Proteomic Processes Related to the Cultivar-Dependent Cadmium Accumulation of Amaranthus gangeticus.
- Author
-
He CT, Zhou YH, Huang YY, Fu HL, Wang XS, Gong FY, Tan X, and Yang ZY
- Subjects
- Biological Transport, Cadmium analysis, Cadmium chemistry, Plant Roots chemistry, Plant Stems chemistry, Soil Pollutants metabolism, Species Specificity, Amaranthus metabolism, Cadmium metabolism, Proteomics
- Abstract
To deal with the Cd contaminant of agricultural soil, pollution-safe cultivar (PSC) is developed to minimize the Cd accumulation risk in crops. The present study aimed to investigate the different proteomic responses related to Cd accumulation in different tissues between two Amaranthus gangeticus cultivars, Pen and Nan. A significantly higher Cd accumulation in Pen than in Nan was unraveled, especially in shoot. The proportions of soluble Cd in root and stem of Nan were significantly lower than those of Pen, implying lower Cd transportation from root to shoot in Nan. Higher contents of NaCl-extracted Cd in Pen than in Nan were probably attributed to the enhancement of GSH related metabolism in Pen, which activated the transportation of Cd from root to shoot. Alteration of other proteins involved in Cd detoxification and energy production also demonstrated that Pen had exhibited a stronger tolerance than Nan in dealing with Cd stress. Thus, differences in the proteomic processes associated with biochemical differences between the two typical cultivars suggested a cultivar-dependent capacity of Cd tolerance and accumulation in amaranth for the first time.
- Published
- 2018
- Full Text
- View/download PDF
7. Comparative Transcriptome Analysis of Two Ipomoea aquatica Forsk. Cultivars Targeted To Explore Possible Mechanism of Genotype-Dependent Accumulation of Cadmium.
- Author
-
Huang YY, Shen C, Chen JX, He CT, Zhou Q, Tan X, Yuan JG, and Yang ZY
- Subjects
- Cadmium analysis, Food Contamination analysis, Gene Expression Profiling, Genotype, Ipomoea chemistry, Ipomoea metabolism, Plant Proteins metabolism, Soil Pollutants analysis, Soil Pollutants metabolism, Transcriptome, Cadmium metabolism, Ipomoea genetics, Plant Proteins genetics
- Abstract
A low-shoot-Cd (QLQ) and a high-shoot-Cd cultivar (T308) of water spinach (Ipomoea aquatica Forsk.) were used to investigate molecular mechanism of the genotype difference in cadmium (Cd) accumulation. RNA-Seq under 9 and 72 h cadmium exposures (5 mg L(-1)) were undertaken to explore Cd induced genotype differences in molecular processes. In total, 253 747 540 clean reads were assembled into 57 524 unigenes. Among them, 6136 and 10 064 unigenes were differentially expressed in QLQ and T308, respectively. Cell wall biosynthesis genes, such as GAUT and laccase, and three Cd efflux genes (Nramp5, MATE9, and YSL7) had higher expression levels in QLQ, while the genes in sulfur and glutathione metabolism pathway, e.g., sulfate transporter and cysteine synthase, showed higher expression levels in T308. These findings would be useful for further understanding of the mechanisms related to genotype-dependent Cd accumulation and developing the molecular assisted screening and breeding of low-shoot-Cd cultivars for water spinach.
- Published
- 2016
- Full Text
- View/download PDF
8. Comparative Transcriptome Analysis between Low- and High-Cadmium-Accumulating Genotypes of Pakchoi (Brassica chinensis L.) in Response to Cadmium Stress.
- Author
-
Zhou Q, Guo JJ, He CT, Shen C, Huang YY, Chen JX, Guo JH, Yuan JG, and Yang ZY
- Subjects
- Gene Expression Profiling, Genotype, Transcriptome, Brassica metabolism, Cadmium metabolism
- Abstract
To reduce cadmium (Cd) pollution of food chains, screening and breeding of low-Cd-accumulating cultivars are the focus of much study. Two previously identified genotypes, a low-Cd-accumulating genotype (LAJK) and a high-Cd-accumulating genotype (HAJS) of pakchoi (Brassica chinesis L.), were stressed by Cd (12.5 μM) for 0 h (T0), 3 h (T3) and 24 h (T24). By comparative transcriptome analysis for root tissue, 3005 and 4343 differentially expressed genes (DEGs) were identified in LAJK at T3 (vs T0) and T24 (vs T3), respectively, whereas 8677 and 5081 DEGs were detected in HAJS. Gene expression pattern analysis suggested a delay of Cd responded transcriptional changes in LAJK compared to HAJS. DEG functional enrichments proposed genotype-specific biological processes coped with Cd stress. Cell wall biosynthesis and glutathione (GSH) metabolism were found to involve in Cd resistance in HAJS, whereas DNA repair and abscisic acid (ABA) signal transduction pathways played important roles in LAJK. Furthermore, the genes participating in Cd efflux such as PDR8 were overexpressed in LAJK, whereas those responsible for Cd transport such as YSL1 were more enhanced in HAJS, exhibiting different Cd transport processes between two genotypes. These novel findings should be useful for molecular assisted screening and breeding of low-Cd-accumulating genotypes for pakchoi.
- Published
- 2016
- Full Text
- View/download PDF
9. Polychlorinated Biphenyls (PCBs) in Human Hair and Serum from E-Waste Recycling Workers in Southern China: Concentrations, Chiral Signatures, Correlations, and Source Identification.
- Author
-
Zheng J, Yu LH, Chen SJ, Hu GC, Chen KH, Yan X, Luo XJ, Zhang S, Yu YJ, Yang ZY, and Mai BX
- Subjects
- China, Dust analysis, Environmental Pollutants blood, Female, Hair chemistry, Humans, Male, Electronic Waste, Occupational Exposure analysis, Polychlorinated Biphenyls blood, Recycling
- Abstract
Hair is increasingly used as a biomarker for human exposure to persistent organic pollutants (POPs). However, the internal and external sources of hair POPs remain a controversial issue. This study analyzed polychlorinated biphenyls (PCBs) in human hair and serum from electronic waste recycling workers. The median concentrations were 894 ng/g and 2868 ng/g lipid in hair and serum, respectively. The PCB concentrations in male and female serum were similar, while concentrations in male hair were significantly lower than in female hair. Significant correlations between the hair and serum PCB levels and congener profiles suggest that air is the predominant PCB source in hair and that hair and blood PCB levels are largely dependent on recent accumulation. The PCB95, 132, and 183 chiral signatures in serum were significantly nonracemic, with mean enantiomer fractions (EFs) of 0.440-0.693. Nevertheless, the hair EFs were essentially racemic (mean EFs = 0.495-0.503). Source apportionment using the Chemical Mass Balance model also indicated primary external PCB sources in human hair from the study area. Air, blood, and indoor dust are responsible for, on average, 64.2%, 27.2%, and 8.79% of the hair PCBs, respectively. This study evidenced that hair is a reliable matrix for monitoring human POP exposure.
- Published
- 2016
- Full Text
- View/download PDF
10. Polybrominated Diphenyl Ethers (PBDEs) in paired human hair and serum from e-waste recycling workers: source apportionment of hair PBDEs and relationship between hair and serum.
- Author
-
Zheng J, Chen KH, Luo XJ, Yan X, He CT, Yu YJ, Hu GC, Peng XW, Ren MZ, Yang ZY, and Mai BX
- Subjects
- Adult, Body Burden, China, Dust analysis, Electronic Waste analysis, Environmental Monitoring methods, Female, Halogenated Diphenyl Ethers blood, Humans, Male, Middle Aged, Occupational Exposure analysis, Polybrominated Biphenyls analysis, Polybrominated Biphenyls blood, Recycling, Electronic Waste adverse effects, Hair chemistry, Halogenated Diphenyl Ethers analysis, Occupational Exposure adverse effects
- Abstract
Human hair has been widely used as a bioindicator for human persistent organic pollutants (POPs) exposure, but studies on the sources of hair POPs and the relationship between hair and body burden are limited. This study analyzed the possible source apportionment of hair PBDEs and examined the relationship between PBDE concentrations in paired hair and serum from e-waste recycling workers. Using the ratio of BDE 99/47 and BDE 209/207 as indices, we calculated that only 15% of the highly brominated congeners (nona- and deca-BDE congeners) comes from exogenous (external) exposure for both female and male hair, but an average of 64% and 55% of the lower-brominated congeners (tetra- to penta-BDE congeners) come from exogenous exposure for female and male hair, respectively. The higher contribution of exogenous exposure for less-brominated congeners could be related to their relatively lower log KOW and higher volatility than higher-brominated congeners, which make them more readily to evaporate from dust and then to be adsorbed on hair. Higher hair PBDE levels and higher exogenous exposure of less-brominated congeners in females than in males can be attributed to a longer exogenous exposure time for females than males. Significant positive relationships were found in tri- to hepta-BDE congeners (BDE 28, 47, 66, 85, 100, 153, 154, and 183) (R = 0.36-0.55, p < 0.05) between hair and serum, but this relationship was not found for octa- to deca-BDE. Difference in the half-lives between highly brominated congeners and less-brominated congeners could be a reason. This result also implied that we should treat the results of correlation analyses between hair and other organs cautiously.
- Published
- 2014
- Full Text
- View/download PDF
11. Dechlorane Plus in human hair from an e-waste recycling area in South China: comparison with dust.
- Author
-
Zheng J, Wang J, Luo XJ, Tian M, He LY, Yuan JG, Mai BX, and Yang ZY
- Subjects
- China, Environmental Monitoring, Environmental Pollutants analysis, Flame Retardants analysis, Humans, Hydrocarbons, Chlorinated analysis, Polycyclic Compounds analysis, Recycling, Waste Management, Dust analysis, Electronic Waste analysis, Environmental Pollutants metabolism, Flame Retardants metabolism, Hair metabolism, Hydrocarbons, Chlorinated metabolism, Polycyclic Compounds metabolism
- Abstract
Dechlorane Plus (DP) and a dechlorination product, 1,6,7,8,9,14,15,16,17,17,18-octadeca-7,15-diene (anti-Cl(11)-DP), were measured in human hair and indoor dust collected from an e-waste recycling area and two control areas (rural and urban) in South China. DP was detected in hair and dust samples at concentrations ranging from 0.02-58.32 ng/g and 2.78-4197 ng/g, respectively. anti-Cl(11)-DP, mainly detected in human hair and dust samples from the e-waste recycling area, ranged from nd (nondetected) to 0.23 ng/g in hair and from nd to 20.22 ng/g in dust. Average values of anti-DP fractional abundance (f(anti) ratio) in hair of e-waste dismantling workers (0.55 ± 0.11) and dust from e-waste recycling workshops (0.54 ± 0.15) were significantly lower than those in other groups (0.62-0.76 means for hair and 0.66-0.76 means for dust). Significantly positive correlation between DP concentrations in dust and hair and similarity in f(anti) ratios between hair and dust suggest that ingestion of dust comprise one of the major routes for DP exposure. Significantly positive relationships were also observed between anti-Cl(11)-DP and anti-DP for both hair and dust samples with similar regression line slopes. The ratios of anti-Cl(11)-DP to anti-DP between hair and dust show no significant difference. These results suggest that anti-Cl(11)-DP in the human body is likely accumulated from the environmental matrix and not formed from biotransformation of the parent DP.
- Published
- 2010
- Full Text
- View/download PDF
12. Residues of polybrominated diphenyl ethers in frogs (Rana limnocharis) from a contaminated site, South China: tissue distribution, biomagnification, and maternal transfer.
- Author
-
Wu JP, Luo XJ, Zhang Y, Chen SJ, Mai BX, Guan YT, and Yang ZY
- Subjects
- Animals, China, Female, Male, Maternal-Fetal Exchange, Tissue Distribution, Tissue Extracts chemistry, Environmental Pollutants chemistry, Environmental Pollutants metabolism, Halogenated Diphenyl Ethers chemistry, Halogenated Diphenyl Ethers metabolism, Pesticide Residues chemistry, Pesticide Residues metabolism, Ranidae metabolism
- Abstract
Environmental pollutants are suspected to be a cause of global declines in amphibian populations, but few data are available on the bioaccumulation of polybrominated diphenyl ethers (PBDEs) in amphibians. To examine the tissue distribution, biomagnification potential, and maternal transfer of PBDEs in frogs, eighteen PBDE congeners were measured in the muscle, liver, and egg tissues of rice frogs (Rana limnocharis) and insects collected from an electronic waste (e-waste) recycling site in South China. PBDE levels in the frogs ranged from 0.63 to 11.6, 4.57 to 56.2, and 10.7 to 125 ng/g wet wt in the muscles, livers, and eggs, respectively. The frogs exhibited a unique congener profile, compared to those in aquatic and terrestrial species, with BDEs 99, 153, 183, 209, and 47 as the dominant congeners, intermediating between aquatic and terrestrial species. Most of the PBDE congeners in general showed higher affinity to liver than to muscle tissue. Except for BDEs 28, 47, 66, 138, and 206, the average biomagnification factors (BMFs) for all PBDE congeners were greater than 1.0, providing clear evidence of their biomagnification from insects to frogs. A parabolic relationship between log BMFs and bromine atom numbers or log Kow of PBDEs was observed, with the maximum BMF values for PBDEs with 6 bromine atoms (or at a log K(ow) of approximately 8.0). Relatively higher levels of 3-MeO-BDE 47 were found in male frogs, suggesting that male frogs in the present study might have higher metabolic capacity for PBDEs compared to female frogs. The ratio of levels in egg/female liver, indicating mother-to-egg transfer capacity, increased with increasing bromine atom numbers up to 7 and then declined as the bromine atom numbers rose. This indicated that the physicochemical properties of the congeners (e.g., K(ow), molecular sizes, and structures), resulting in different affinities to transport proteins, might impact their maternal transfer in frogs.
- Published
- 2009
- Full Text
- View/download PDF
13. Persistent halogenated compounds in waterbirds from an e-waste recycling region in South China.
- Author
-
Luo XJ, Zhang XL, Liu J, Wu JP, Luo Y, Chen SJ, Mai BX, and Yang ZY
- Subjects
- Animals, China, Environmental Pollutants analysis, Geography, Muscles metabolism, Polybrominated Biphenyls analysis, Principal Component Analysis, Rivers chemistry, Species Specificity, Charadriiformes metabolism, Conservation of Natural Resources, Electronics, Environmental Monitoring, Hydrocarbons, Halogenated analysis, Industrial Waste
- Abstract
Persistent halogenated compounds (PHCs), such as dichlorodiphenyltrichloroethane and its metabolites (DDTs), hexachlorocyclohexane isomers (HCHs), polychlorinated biphenyls (PCBs), polybrominated diphenyl ethers (PBDEs), decabromodiphenylethane (DBDPE), and polybrominated biphenyl 153 (PBB 153), were quantified in muscles of five waterbird species collected from an extensive e-waste recycling region in the Pearl River Delta, South China. PCBs, at concentrations up to 1,400,000 ng/g lipid, were the dominant contaminants contributing to 80%-90% of PHCs. PBDEs and organochlorine pesticides (sum of DDTs and HCHs) contributed approximately equally to total PHCs with median concentrations ranging from 37-2200 and 530-4300 ng/g lipid, respectively. This contaminant distribution pattern was different from those acquired by most studies conducted in other regions. The concentrations of PCBs and PBDEs in Chinese-pond heron from the present study were higher than those from most other previous studies with birds having similar trophic levels. The extensive e-waste recycling activities were probably the cause of the elevated PCB and PBDE levels in the bird samples. The median concentrations of PBB 153 and DBDPE ranged from 3-140 and 10-176 ng/g lipid, respectively. The frequent detection and high concentrations of DBDPE in piscivorous birds implicate a potential environmental concern for this "new" brominated flame retardant. Additionally, the interspecies differences in the levels of contaminants and species-specific PBDE congener patterns were also elucidated in the present study.
- Published
- 2009
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.