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Examines the effect of temperature, monovalent cations, and allosteric ligand on the catalytic properties of tryptophan synthase alpha2beta2 complex and beta2 subunit. Creation of nonlinear Arrhenius plots; Inverse relation of activation energy with temperature; Effect of allosteric ligand and monovalent cations on the balance between open and closed forms.

Characterizes the conformational transitions that regulate the activity and specificity of the tryptophan synthase alpha2beta2 complex. Determination of effects of low concentrations of guanidine hydrochloride and of urea; Reactions with L-serine or beta-chloro-L-alanine in the presence or absence of indole; Stabilization of aldimine by nonionic urea.