1. Paper Spray and Extraction Spray Mass Spectrometry for the Direct and Simultaneous Quantification of Eight Drugs of Abuse in Whole Blood
- Author
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Yue Ren, Nicholas E. Manicke, Ryan D. Espy, Arian C. van Asten, Zheng Ouyang, S.F. Teunissen, R. Graham Cooks, and Analytical Chemistry and Forensic Science (HIMS, FNWI)
- Subjects
Paper ,Detection limit ,Drugs of abuse ,Chromatography ,Illicit Drugs ,010405 organic chemistry ,Chemistry ,010401 analytical chemistry ,Extraction (chemistry) ,MDMA ,Tandem mass spectrometry ,Mass spectrometry ,01 natural sciences ,Mass Spectrometry ,0104 chemical sciences ,Analytical Chemistry ,Limit of Detection ,medicine ,Humans ,Sample preparation ,Whole blood ,medicine.drug - Abstract
Determination of eight drugs of abuse in blood has been performed using paper spray or extraction spray mass spectrometry in under 2 min with minimal sample preparation. A method has been optimized for quantification of amphetamine, methamphetamine, 3,4-methylenedioxyamphetamine (MDA), 3,4-methylenedioxy-N-methylamphetamine (MDMA), 3,4-methylenedioxy-N-ethylamphetamine (MDEA), morphine, cocaine, and Delta 9-tetrahydrocannabinol (THC) from a single blood spot. Sample to sample variations of 1-5% relative standard deviation were achieved using stable isotope-labeled internal standards and tandem mass spectrometry. Limits of detection for all drugs were below typical physiological and toxicological levels. Paper spray and extraction spray each used less than 10 mu L of whole blood. These methods exhibit the potential for performing rapid and high-throughput assays for selective on-site multicompound quantitative screening of illicit drugs.
- Published
- 2014