1. Enzymic determination of carbon-14-labeled L-alanine in biological samples
- Author
-
Francisca Serra, Antoni Pons, and Andreu Palou
- Subjects
Alanine ,chemistry.chemical_classification ,Elution ,Radiochemistry ,Amberlite ,Rats ,Analytical Chemistry ,Amino acid ,chemistry.chemical_compound ,Alanine Dehydrogenase ,chemistry ,Glycine ,Glycerol ,Animals ,Indicators and Reagents ,Carbon-14 ,Amino Acid Oxidoreductases ,Carbon Radioisotopes ,Pyruvic acid - Abstract
A method for determination of L-alanine-specific radioactivity in biological samples is presented. This method is based on the specific enzymatic transformation of L-alanine to pyruvic acid hydrazone catalyzed by the enzyme L-alanine dehydrogenase, formation of the pyruvic acid 2,4-dinitrophenylhydrazone derivative, and quantitative trapping in Amberlite XAD-7 columns, followed by radioactivity counting of the lipophilic eluate. No interferences from other UC-labeled materials such as D-glucose, glycerol, L-lactate, L-serine, L-glutamate, L-phenylalanine, glycine, L-leucine, and L-arginine were observed. This inexpensive and high-speed method is applicable to the simultaneous determination of L-alanine-specific radioactivity for a large number of samples.
- Published
- 1987
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