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Your search keyword '"Harsha Bajaj"' showing total 2 results
Start Over Author "Harsha Bajaj" Publisher american chemical society (acs)
2 results on '"Harsha Bajaj"'

2. Antibiotic Uptake through Membrane Channels: Role of Providencia stuartii OmpPst1 Porin in Carbapenem Resistance

Author

Jean-Marie Pagès, Kozhinjampara R. Mahendran, Jean-Michel Bolla, Chady Nasrallah, Jacques-Phillippe Colletier, Anne Davin-Regli, Harsha Bajaj, Que-Tien Tran, and Mathias Winterhalter

Subjects
Imipenem, medicine.drug_class, Lipid Bilayers, Antibiotics, Porins, Microbial Sensitivity Tests, Providencia, Drug resistance, Biology, Biochemistry, Meropenem, Microbiology, Drug Resistance, Bacterial, polycyclic compounds, medicine, Liposome, Providencia stuartii, Cell Membrane, Electric Conductivity, biochemical phenomena, metabolism, and nutrition, Permeation, bacterial infections and mycoses, biology.organism_classification, Liposomes, Porin, bacteria, Thienamycins, Bacterial Outer Membrane Proteins, medicine.drug
Abstract

The role of major porin OmpPst1 of Providencia stuartii in antibiotic susceptibility for two carbapenems is investigated by combining high-resolution conductance measurements, liposome swelling, and microbiological assays. Reconstitution of a single OmpPst1 into a planar lipid bilayer and measuring the ion current, in the presence of imipenem, revealed a concentration-dependent decrease in conductance, whereas meropenem produced well-resolved short ion current blockages. Liposome swelling assays suggested a small flux of imipenem in contrast to a rapid permeation of meropenem. The lower antibiotic susceptibility of P. stuartii to imipenem compared to meropenem correlated well with the decreased level of permeation of the former through the OmpPst1 channel.

Published
2012
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