1. Detecting Single Stranded DNA with a Solid State Nanopore
- Author
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Bradley Ledden, Jiali Li, Marc Gershow, Daniel Fologea, David S. McNabb, and Jene Andrew Golovchenko
- Subjects
Nanostructure ,Chemistry ,Mechanical Engineering ,Detector ,Solid-state ,DNA, Single-Stranded ,Bioengineering ,General Chemistry ,Condensed Matter Physics ,Article ,Micrometre ,Nanopore ,chemistry.chemical_compound ,Crystallography ,Chemical physics ,Electrochemistry ,Nanotechnology ,Nucleic Acid Conformation ,Molecule ,General Materials Science ,DNA - Abstract
Voltage biased solid-state nanopores are used to detect and characterize individual single stranded DNA molecules of fixed micrometer length by operating a nanopore detector at pH values greater than approximately 11.6. The distribution of observed molecular event durations and blockade currents shows that a significant fraction of the events obey a rule of constant event charge deficit (ecd) indicating that they correspond to molecules translocating through the nanopore in a distribution of folded and unfolded configurations. A surprisingly large component is unfolded. The result is an important milestone in developing solid-state nanopores for single molecule sequencing applications.
- Published
- 2005
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