1. Expression and characterization of human lactoferrin in yeast Saccharomyces cerevisiae
- Author
-
Qianwa. Liang and Tom Richardson
- Subjects
biology ,Lactoferrin ,Saccharomyces cerevisiae ,Heterologous ,General Chemistry ,biology.organism_classification ,Yeast ,law.invention ,Invertase ,Biochemistry ,law ,Complementary DNA ,Recombinant DNA ,biology.protein ,Secretion ,General Agricultural and Biological Sciences - Abstract
Lactoferrin (LF) has certain chemical and biological properties that are significant to the dairy food industry. To study the relationship between protein structure and functionality of LF with the aim of increasing the thermostability of LF, we established a yeast system for heterologous expressionof cloned human LF cDNA. Human LF was successfully synthesized in yeast cells by placing the cloned cDNA under the regulation of yeast chelatin promoter. Both human LF and yeast invertase secretion signal sequences were used to direct the secretion of recombinant human LF synthesized in yeast cells
- Published
- 1993
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