1. Catestatin Inhibits Obesity-Induced Macrophage Infiltration and Inflammation in the Liver and Suppresses Hepatic Glucose Production Leading to Improved Insulin Sensitivity
- Author
-
Nai-Wen Chi, Wei Ying, Zhenqi Zhou, Nicholas J. G. Webster, Gautam Bandyopadhyay, Sumana Mahata, Joshua Wollam, Angelo Corti, Sushil K. Mahata, Rafael Mayoral, Jessica Vu, Ying, Wei, Mahata, Sumana, Bandyopadhyay, Gautam K., Zhou, Zhenqi, Wollam, Joshua, Vu, Jessica, Mayoral, Rafael, Chi, Nai-Wen, Webster, Nicholas J. G., Corti, Angelo, and Mahata, Sushil K.
- Subjects
0301 basic medicine ,Male ,Macrophage ,medicine.medical_treatment ,Endocrinology, Diabetes and Metabolism ,Type 2 diabetes ,Cardiovascular ,Medical and Health Sciences ,Oral and gastrointestinal ,Mice ,0302 clinical medicine ,Peptide Fragment ,2.1 Biological and endogenous factors ,Insulin ,Aetiology ,Mice, Knockout ,biology ,Chemistry ,Liver Disease ,Diabetes ,Chromogranin A ,medicine.anatomical_structure ,Liver ,030220 oncology & carcinogenesis ,Kupffer Cell ,medicine.symptom ,Infiltration (medical) ,Gluconeogenesi ,medicine.medical_specialty ,Kupffer Cells ,Knockout ,Inflammation ,Carbohydrate metabolism ,03 medical and health sciences ,Endocrinology & Metabolism ,Insulin resistance ,Internal medicine ,medicine ,Internal Medicine ,Animals ,Obesity ,Metabolic and endocrine ,Nutrition ,Animal ,Monocyte ,Macrophages ,Gluconeogenesis ,medicine.disease ,Glucagon ,Lipid Metabolism ,Hormone ,Hormones ,Peptide Fragments ,030104 developmental biology ,Endocrinology ,Glucose ,Metabolism ,Gene Expression Regulation ,biology.protein ,Insulin Resistance ,Digestive Diseases ,human activities - Abstract
The activation of Kupffer cells (KCs) and monocyte-derived recruited macrophages (McMΦs) in the liver contributes to obesity-induced insulin resistance and type 2 diabetes. Mice with diet-induced obesity (DIO mice) treated with chromogranin A peptide catestatin (CST) showed several positive results. These included decreased hepatic/plasma lipids and plasma insulin, diminished expression of gluconeogenic genes, attenuated expression of proinflammatory genes, increased expression of anti-inflammatory genes in McMΦs, and inhibition of the infiltration of McMΦs resulting in improvement of insulin sensitivity. Systemic CST knockout (CST-KO) mice on normal chow diet (NCD) ate more food, gained weight, and displayed elevated blood glucose and insulin levels. Supplementation of CST normalized glucose and insulin levels. To verify that the CST deficiency caused macrophages to be very proinflammatory in CST-KO NCD mice and produced glucose intolerance, we tested the effects of (sorted with FACS) F4/80+Ly6C− cells (representing KCs) and F4/80−Ly6C+ cells (representing McMΦs) on hepatic glucose production (HGP). Both basal HGP and glucagon-induced HGP were markedly increased in hepatocytes cocultured with KCs and McMΦs from NCD-fed CST-KO mice, and the effect was abrogated upon pretreatment of CST-KO macrophages with CST. Thus, we provide a novel mechanism of HGP suppression through CST-mediated inhibition of macrophage infiltration and function.
- Published
- 2018