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By utilizing resistant plants, viral diseases control can be done indirectly to reduce the source of inoculum. In previous studies, double-crosses approach of genetically engineered (GE) tomato lines were carried out to obtain lines which have resistance to tomato yellow leaf curl virus (TYLCV) and cucumber mosaic virus (CMV). The aim of this study was to conduct molecular analysis and evaluation on fruit performance of two GE tomato lines (F8) resistant to TYLCV and CMV. The plant genetic materials used were two GE tomato lines, namely F8-IC-CL-34-2-9-E2-2-T2E11 and F8-IC-CL-34-2-9-E2-2-T2E14, and their three parental genotypes, namely CL 6046 (non-GE, susceptible to TYLCV and CMV), FLA456 (non-GE, resistant to TYLCV), and R8-110-11/CMV-A (GE, resistant to CMV). The study was performed in a single-factor completely randomized design. The result of molecular analysis showed that the two GE tomato lines contained transgene for resistance to CMV similar to GE parental genotype. Based on fruit performance, the two GE tomato lines showed equivalence in almost all characters when compared to their parental genotypes. The round-shaped fruit of F8-IC-CL-34-2-9-E2-2-T2E11 line was similar to non-GE parental genotype FLA456. Meanwhile, F8-IC-CL-34-2-9-E2-2-T2E14 line had equivalence for all of the observed fruit size characters with non-GE parental genotype CL 6046, including its oval-shaped fruit. F8-IC-CL-34-2-9-E2-2-T2E11 line was in commensurate with one or all three of its parental genotypes for each observed character related to production. Results of this study also indicated that the F8-IC-CL-34-2-9-E2-2-T2E14 line had higher yield potential compared to the three parental genotypes. [ABSTRACT FROM AUTHOR]

Seed storage conditions and the availability of shallot seeds are critical factors in the development of shallots. The main objective of this study was to determine the ideal storage conditions for several varieties of shallot (Allium cepa var aggregatum L.) on the physical quality of seeds, growth and yield of shallots. This research was conducted at the Experimental Garden, Indonesian Vegetables Research Institute, West Java. This research was conducted using a split-plot design with two replications. The main plot was a storage room which consists of three storage conditions, namely the growth chamber (temperature 1-2°C), shallot storage room (temperature 29-30°C), and room temperature (22.5°C). The subplot consists of seven shallot varieties, namely Bima Brebes, Mentes, Trisula, Pancasona, Pikatan, Kuning and Katumi. Firstly, the seed is stored in the storage area and afterwards is planted in the field. Observation parameters included seed quality after storage, growth components and yield components. The results showed the interaction effect of seed storage conditions and cultivars on the number of seeds that grew (%) on day 5 DAP and the dry weight of bulbs per hectare (tons). Kuning cultivar stored in the growth chamber (1-2°C) produced the highest dry weight (9.08 tons) compared to all combinations of storage space and varieties unless stored in shallot storage (29-30°C). [ABSTRACT FROM AUTHOR]