1. Fluorescence diffuse optical tomography using upconverting nanoparticles
- Author
-
Johan Axelsson, Can T. Xu, and Stefan Andersson-Engels
- Subjects
Materials science ,Photoluminescence ,Physics and Astronomy (miscellaneous) ,Tissue imaging ,Atom and Molecular Physics and Optics ,Nanoparticle ,phantoms ,optical tomography ,nanobiotechnology ,Imaging phantom ,Optics ,medicine ,Upconverting nanoparticles ,Optical tomography ,medicine.diagnostic_test ,business.industry ,Resolution (electron density) ,ytterbium ,Fluorescence ,thulium ,Diffuse optical imaging ,Autofluorescence ,Quantum dot ,yttrium compounds ,photoluminescence ,nanoparticles ,biological tissues ,fluorescence ,sodium compounds ,business - Abstract
Fluorescence diffuse optical tomography (FDOT) can provide important information in biomedical studies. In this ill-posed problem, suppression of background tissue autofluorescence is of utmost importance. We report a method for autofluorescence-insensitive FDOT using nonlinear upconverting nanoparticles (NaYF4:Yb3+/Tm3+) in a tissue phantom under excitation intensities well below tissue-damage thresholds. Even with the intrinsic autofluorescence from the phantom only, the reconstruction of the nanoparticles is of much better quality than the reconstruction of a Stokes-shifting dye. In addition, the nonlinear power dependence leads to more confined reconstructions and may increase the resolution in FDOT.
- Published
- 2009