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This study evaluates the feasibility of applying the Estimating the Circulation and Climate of the Ocean (ECCO) data assimilation (DA) framework to a submesoscale-resolving model [Massachusetts Institute of Technology General Circulation Model (MITgcm), at 1-km grid resolution]. This is in preparation for future studies to understand and assimilate the novel swath measurements of sea surface height from the Surface Water and Ocean Topography (SWOT) satellite mission. The model domain is centered at the SWOT calibration/validation (CalVal) site, located 300 km offshore of Monterey Bay, California. We assimilate vertical profiles of temperature and salinity from a linear array of three SWOT prelaunch CalVal moorings, between September and December 2019. Two model solutions are analyzed: 1) a nonassimilating forward simulation termed "first-guess" and 2) an optimized solution that assimilates the in situ observations. Both runs are nested within the global 1/12° Hybrid Coordinate Ocean Model that uses Navy Coupled Ocean Data Assimilation (HYCOM/NCODA) analysis. We evaluate the performance by comparing the two model solutions against assimilated and withheld in situ observations. We show that by assimilating hydrographic data, the model performance over the first-guess solution is improved with an error-to-observation reduction of 30%–45% for temperature, 14%–41% for salinity, and 44%–56% for steric height. Over the study period, the average steric height error at the three assimilated moorings was 1.27 cm for the optimized solution versus 2.6 cm for the first-guess solution. A comparison to withheld glider observations shows that the optimized solution outperforms the first-guess solution with an error-to-observation reduction of approximately 38% in steric height (1.5 versus 2.4 cm). This study indicates, for the first time, that the MITgcm–ECCO framework can be successfully applied to the reconstruction of submesoscale ocean variability, via the nesting of a high-resolution regional domain into a global outer domain. [ABSTRACT FROM AUTHOR]