1. Super-resolution two-photon microscopy via scanning patterned illumination.
- Author
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Urban, Ben E., Ji Yi, Siyu Chen, Biqin Dong, Yongling Zhu, DeVries, Steven H., Backman, Vadim, and Zhang, Hao F.
- Subjects
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MICROSCOPY , *HIGH resolution imaging , *CELL imaging , *CYTOSKELETON , *TWO-photon-spectroscopy , *RETINA analysis - Abstract
We developed two-photon scanning patterned illumination microscopy (2P-SPIM) for super-resolution two-photon imaging. Our approach used a traditional two-photon microscopy setup with temporally modulated excitation to create patterned illumination fields. Combing nine different illuminations and structured illumination reconstruction, super-resolution imaging was achieved in two-photon microscopy. Using 2P-SPIM we achieved a lateral resolution of 141 nm, which represents an improvement by a factor of 1.9 over the corresponding diffraction limit. We further demonstrated super-resolution cellular imaging by 2P-SPIM to image actin cytoskeleton in mammalian cells and three-dimensional imaging in highly scattering retinal tissue. [ABSTRACT FROM AUTHOR]
- Published
- 2015
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