10 results on '"Hashimoto, Seiichi"'
Search Results
2. Inhibitory role of REV-ERBα in the expression of bone morphogenetic protein gene family in rat uterus endometrium stromal cells
- Author
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Tasaki, Hirotaka, primary, Zhao, Lijia, additional, Isayama, Keishiro, additional, Chen, Huatao, additional, Yamauchi, Nobuhiko, additional, Shigeyoshi, Yasufumi, additional, Hashimoto, Seiichi, additional, and Hattori, Masa-aki, additional
- Published
- 2015
- Full Text
- View/download PDF
3. Downregulation of core clock gene Bmal1 attenuates expression of progesterone and prostaglandin biosynthesis-related genes in rat luteinizing granulosa cells
- Author
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Chen, Huatao, primary, Zhao, Lijia, additional, Kumazawa, Makoto, additional, Yamauchi, Nobuhiko, additional, Shigeyoshi, Yasufumi, additional, Hashimoto, Seiichi, additional, and Hattori, Masa-aki, additional
- Published
- 2013
- Full Text
- View/download PDF
4. FSH induces the development of circadian clockwork in rat granulosa cells via a gap junction protein Cx43-dependent pathway
- Author
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Chen, Huatao, primary, Zhao, Lijia, additional, Chu, Guiyan, additional, Kito, Gakushi, additional, Yamauchi, Nobuhiko, additional, Shigeyoshi, Yasufumi, additional, Hashimoto, Seiichi, additional, and Hattori, Masa-aki, additional
- Published
- 2013
- Full Text
- View/download PDF
5. Contribution of FSH and triiodothyronine to the development of circadian clocks during granulosa cell maturation
- Author
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Chu, Guiyan, primary, Misawa, Izumi, additional, Chen, Huatao, additional, Yamauchi, Nobuhiko, additional, Shigeyoshi, Yasufumi, additional, Hashimoto, Seiichi, additional, and Hattori, Masa-aki, additional
- Published
- 2012
- Full Text
- View/download PDF
6. Removal of Rev-erbα inhibition contributes to the prostaglandin G/H synthase 2 expression in rat endometrial stromal cells.
- Author
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Isayama K, Zhao L, Chen H, Yamauchi N, Shigeyoshi Y, Hashimoto S, and Hattori MA
- Subjects
- 5' Untranslated Regions, ARNTL Transcription Factors antagonists & inhibitors, ARNTL Transcription Factors genetics, ARNTL Transcription Factors metabolism, Animals, Cells, Cultured, Cyclooxygenase 2 genetics, Endometrium cytology, Endometrium enzymology, Female, Nuclear Receptor Subfamily 1, Group D, Member 1 antagonists & inhibitors, Nuclear Receptor Subfamily 1, Group D, Member 1 genetics, Nuclear Receptor Subfamily 1, Group F, Member 1 genetics, Nuclear Receptor Subfamily 1, Group F, Member 1 metabolism, Pregnancy, Prolactin analogs & derivatives, Prolactin genetics, Prolactin metabolism, RNA Interference, RNA, Small Interfering, Rats, Rats, Transgenic, Response Elements, Stromal Cells cytology, Stromal Cells enzymology, Circadian Clocks, Cyclooxygenase 2 metabolism, Endometrium metabolism, Gene Expression Regulation, Enzymologic, Nuclear Receptor Subfamily 1, Group D, Member 1 metabolism, Placentation, Stromal Cells metabolism
- Abstract
The rhythmic expression of clock genes in the uterus is attenuated during decidualization. This study focused on Ptgs2, which is essential for decidualization, as a putative clock-controlled gene, and aimed to reveal the functions of clock genes in relation to Ptgs2 during decidualization. We compared the transcript levels of clock genes in the rat uterus on days 4.5 (D4.5) and 6.5 of pregnancy. The transcript levels of clock genes (Per2, Bmal1, Rorα, and Rev-erbα) had decreased at implantation sites on day 6.5 (D6.5e) compared with those on D4.5, whereas Ptgs2 transcripts had increased on D6.5e. Similar observations of Rev-erbα and Ptgs2 were also obtained in the endometrium on D6.5e by immunohistochemistry. In the decidual cells induced by medroxyprogesterone and 2-O-dibutyryl-cAMP, the rhythmic expression levels of clock genes were attenuated, whereas Ptgs2 transcription was induced. These results indicate that decidualization causes the attenuation of clock genes and the induction of Ptgs2. Furthermore, in the experiment of Bmal1 siRNA, the rhythmic expression of clock genes and Ptgs2 was attenuated by the siRNA. Transcript levels of Ptgs2 and prostaglandin (PG)E₂ production were increased by treatment with the Rev-erbα antagonist, suggesting the contribution of the nuclear receptor Rev-erbα to Ptgs2 expression. Moreover, Rev-erbα knockdown enhanced the induction of Ptgs2 transcription and PGE₂ production by forskolin. Chromatin immunoprecipitation-PCR analysis revealed that Rev-erbα could directly bind to a proximal RORE site of Ptgs2. Collectively, this study demonstrates that the attenuation of the circadian clock, especially its core component Rev-erbα, contributes to the induction of Ptgs2 during decidualization., (Copyright © 2015 the American Physiological Society.)
- Published
- 2015
- Full Text
- View/download PDF
7. Inhibitory role of REV-ERBα in the expression of bone morphogenetic protein gene family in rat uterus endometrium stromal cells.
- Author
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Tasaki H, Zhao L, Isayama K, Chen H, Yamauchi N, Shigeyoshi Y, Hashimoto S, and Hattori MA
- Subjects
- Animals, Cells, Cultured, Female, Gene Expression Regulation, Pregnancy, Rats, Rats, Transgenic, Stromal Cells metabolism, Uterus cytology, Uterus metabolism, Bone Morphogenetic Proteins antagonists & inhibitors, Bone Morphogenetic Proteins biosynthesis, Endometrium cytology, Endometrium metabolism, Nuclear Receptor Subfamily 1, Group D, Member 1 biosynthesis
- Abstract
Uterus circadian rhythms have been implicated in the gestation processes of mammals through entraining of the clock proteins to numerous downstream genes. Bone morphogenetic proteins (BMPs), having clock-controlled regulatory sites in their gene promoters, are expressed in the uterus during decidualization, but the regulation of the Bmp gene expression is poorly understood. The present study was designed to dissect the physiological roles of the uterus oscillators in the Bmp expression using the uterus endometrial stromal cells (UESCs) isolated from Per2-dLuc transgenic rats on day 4.5 of gestation. The in vitro decidualization of UESCs was induced by medroxyprogesterone acetate and 2-O-dibutyryl cAMP. A significant decline of Per2-dLuc bioluminescence activity was induced in decidual cells, and concomitantly, the expression of canonical clock genes was downregulated. Conversely, the expression of the core Bmp genes Bmp2, Bmp4, Bmp6, and Bmp7 was upregulated. In UESCs transfected with Bmal1-specific siRNA, in which Rev-erbα expression was downregulated, Bmp genes, such as Bmp2, Bmp4, and Bmp6 were upregulated. However, Bmp1, Bmp7, and Bmp8a were not significantly affected by Bmal1 silencing. The expression of all Bmp genes was enhanced after treatment with the REV-ERBα antagonist (SR8278), although their rhythmic profiles were differed from each other. The binding of REV-ERBα to the proximal regions of the Bmp2 and Bmp4 promoters was revealed by chromatin immunoprecipitation-PCR analysis. Collectively, these results indicate that the Bmp genes are upregulated by the attenuation of the cellular circadian clock; in particular, its core component REV-ERBα functions as a transcriptional silencer in the Bmp gene family., (Copyright © 2015 the American Physiological Society.)
- Published
- 2015
- Full Text
- View/download PDF
8. Downregulation of core clock gene Bmal1 attenuates expression of progesterone and prostaglandin biosynthesis-related genes in rat luteinizing granulosa cells.
- Author
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Chen H, Zhao L, Kumazawa M, Yamauchi N, Shigeyoshi Y, Hashimoto S, and Hattori MA
- Subjects
- ARNTL Transcription Factors biosynthesis, ARNTL Transcription Factors genetics, Animals, CLOCK Proteins antagonists & inhibitors, CLOCK Proteins biosynthesis, Cells, Cultured, Female, Gene Expression Regulation, Mice, Progesterone biosynthesis, Progesterone genetics, Prostaglandins biosynthesis, Rats, Rats, Transgenic, ARNTL Transcription Factors antagonists & inhibitors, CLOCK Proteins genetics, Down-Regulation genetics, Luteal Cells metabolism, Progesterone antagonists & inhibitors, Prostaglandins genetics
- Abstract
Ovarian circadian oscillators have been implicated in the reproductive processes of mammals. However, there are few reports regarding the detection of ovarian clock-controlled genes (CCGs). The present study was designed to unravel the mechanisms through which CCG ovarian circadian oscillators regulate fertility, primarily using quantitative RT-PCR and RNA interference against Bmal1 in rat granulosa cells. Mature granulosa cells were prepared from mouse Per2-destabilized luciferase (dLuc) reporter gene transgenic rats. A real-time monitoring system of Per2 promoter activity was employed to detect Per2-dLuc oscillations. The cells exposed to luteinizing hormone (LH) displayed clear Per2-dLuc oscillations and a rhythmic expression of clock genes (Bmal1, Per1, Per2, Rev-erbα, and Dbp). Meanwhile, the examined ovarian genes (Star, Cyp19a1, Cyp11a1, Ptgs2, Lhcgr, and p53) showed rhythmic transcript profiles except for Hsd3b2, indicating that these rhythmic expression genes may be CCGs. Notably, Bmal1 small interfering (si)RNA treatment significantly decreased both the amplitude of Per2-dLuc oscillations and Bmal1 mRNA levels compared with nonsilencing RNA treatment in luteinizing granulosa cells. Depletion of Bmal1 by siRNA decreased the transcript levels of clock genes (Per1, Per2, Rev-erbα, and Dbp) and examined ovarian genes (Star, Cyp19a1, Cyp11a1, Ptgs2, Hsd3b2, and Lhcgr). Accordingly, knockdown of Bmal1 also inhibited the synthesis of progesterone and prostaglandin E2, which are associated with crucial reproductive processes. Collectively, these data suggest that ovarian circadian oscillators regulate the synthesis of steroid hormones and prostaglandins through ovarian-specific CCGs in response to LH stimuli. The present study provides new insights into the physiologic significance of Bmal1 related to fertility in ovarian circadian oscillators.
- Published
- 2013
- Full Text
- View/download PDF
9. FSH induces the development of circadian clockwork in rat granulosa cells via a gap junction protein Cx43-dependent pathway.
- Author
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Chen H, Zhao L, Chu G, Kito G, Yamauchi N, Shigeyoshi Y, Hashimoto S, and Hattori MA
- Subjects
- Animals, Cell Differentiation drug effects, Cells, Cultured, Chorionic Gonadotropin metabolism, Circadian Rhythm Signaling Peptides and Proteins biosynthesis, Circadian Rhythm Signaling Peptides and Proteins genetics, Circadian Rhythm Signaling Peptides and Proteins metabolism, Connexin 43 antagonists & inhibitors, Connexin 43 genetics, Dexamethasone pharmacology, Female, GABA-A Receptor Antagonists pharmacology, Gap Junctions drug effects, Genes, Reporter drug effects, Glucocorticoids pharmacology, Granulosa Cells cytology, Granulosa Cells drug effects, Membrane Transport Modulators pharmacology, Mice, Period Circadian Proteins biosynthesis, Period Circadian Proteins genetics, Period Circadian Proteins metabolism, Rats, Rats, Transgenic, Receptors, LH biosynthesis, Receptors, LH genetics, Receptors, LH metabolism, Circadian Clocks drug effects, Connexin 43 metabolism, Follicle Stimulating Hormone metabolism, Gap Junctions metabolism, Granulosa Cells metabolism, Up-Regulation drug effects
- Abstract
The present study was designed to assess the relationship between gap junctions and the maturation of a clock system in rat granulosa cells stimulated by follicle-stimulating hormone (FSH). Immature and mature granulosa cells were prepared by puncturing the ovaries of diethylstilbestrol- and equine chorionic gonadotropin (eCG)-treated mouse Period2 (Per2)-dLuc reporter gene transgenic rats, respectively. Mature granulosa cells exposed to dexamethasone (DXM) synchronization displayed several Per2-dLuc oscillations and a rhythmic expression of clock genes. Intriguingly, we observed clear evidence that the FSH stimulation significantly increased the amplitude of Per2 oscillations in the granulosa cells, which was confirmed by the elevation of the Per2 and Rev-erbα (Nr1d1) mRNA levels. FSH also induced a major phase-advance shift of Per2 oscillations. The mature granulosa cells cultured for 2 days with FSH expressed higher mRNA levels of Per2, Rev-erbα, Bmal1 (Arnt1), Lhcgr, and connexin (Cx) 43 (Gja1) compared with the immature granulosa cells. Consistently, our immunofluorescence results revealed abundant Cx43 protein in antral follicles stimulated with eCG and weak or no fluorescence signal of Cx43 in primary and preantral follicles. Similar results were confirmed by Western blotting analysis. Two gap junction blockers, lindane and carbenoxolone (CBX), significantly decreased the amplitude of Per2 oscillations, which further adhered significant decreases in Per2 and Rev-erbα transcript levels. In addition, both lindane and CBX induced a clear phase-delay shift of Per2 oscillations. These findings suggest that FSH induces the development of the clock system by increasing the expression of Cx43.
- Published
- 2013
- Full Text
- View/download PDF
10. Contribution of FSH and triiodothyronine to the development of circadian clocks during granulosa cell maturation.
- Author
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Chu G, Misawa I, Chen H, Yamauchi N, Shigeyoshi Y, Hashimoto S, and Hattori MA
- Subjects
- Animals, Circadian Rhythm Signaling Peptides and Proteins biosynthesis, Circadian Rhythm Signaling Peptides and Proteins genetics, Culture Media, Serum-Free, Diethylstilbestrol pharmacology, Estrogens, Non-Steroidal pharmacology, Female, Genes, Reporter, Luminescence, Ovary cytology, Ovary growth & development, Period Circadian Proteins biosynthesis, Period Circadian Proteins genetics, RNA biosynthesis, RNA genetics, Rats, Real-Time Polymerase Chain Reaction, Transcription, Genetic physiology, Circadian Rhythm physiology, Follicle Stimulating Hormone physiology, Granulosa Cells physiology, Triiodothyronine physiology
- Abstract
The involvement of FSH and triiodothyronine (T(3)) in circadian clocks was investigated using immature granulosa cells of ovaries during the progress of cell maturation. Granulosa cells were prepared from preantral follicles of mouse Period2 (Per2)-dLuc reporter gene transgenic rats injected subcutaneously with the synthetic nonsteroidal estrogen diethylstilbestrol. Analysis of the cellular clock of the immature granulosa cells was performed partly using a serum-free culture system. Several bioluminescence oscillations of Per2-dLuc promoter activity were generated in the presence of FSH + fetal bovine serum, but not in the presence of either FSH or serum. As revealed by bioluminescence recording and analysis of clock gene expression, the granulosa cells lack the functional cellular clock at the immature stage, although Lhr was greatly expressed during the period of cell maturation. The granulosa cells gained a strong circadian rhythm of bioluminescence during stimulation with FSH, whereas LH reset the cellular clock of matured granulosa cells. During strong circadian rhythms of clock genes, the Star gene showed significant expression in matured granulosa cells. In contrast, T(3) showed an inhibitory effect on the development of the functional cellular clock during the period of cell maturation. These results indicate that FSH provides a cue for the development of the functional cellular clock of the immature granulosa cells, and T(3) blocks the development of the cellular clock.
- Published
- 2012
- Full Text
- View/download PDF
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