1. Injection of autologous bone marrow cells in hyaluronan hydrogel improves cardiac performance after infarction in pigs
- Author
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Patrick C.H. Hsieh, Chien-Hsi Chen, Ming-Yao Chang, and Shoei-Shen Wang
- Subjects
medicine.medical_specialty ,Time Factors ,Swine ,Physiology ,Myocardial Infarction ,Neovascularization, Physiologic ,Infarction ,Injections, Intramuscular ,Transplantation, Autologous ,Peripheral blood mononuclear cell ,Ventricular Function, Left ,Neovascularization ,Cell therapy ,Physiology (medical) ,Internal medicine ,Animals ,Regeneration ,Medicine ,Myocardial infarction ,Hyaluronic Acid ,Bone Marrow Transplantation ,Ventricular Remodeling ,business.industry ,Myocardium ,Hemodynamics ,Cell Differentiation ,Hydrogels ,Stroke Volume ,Recovery of Function ,medicine.disease ,Autologous bone ,Coronary Vessels ,Myocardial Contraction ,Disease Models, Animal ,medicine.anatomical_structure ,Cardiology ,Swine, Miniature ,Collagen ,Bone marrow ,medicine.symptom ,Cardiology and Cardiovascular Medicine ,business ,Ischemic heart - Abstract
Intramyocardial injection of bone marrow mononuclear cells (MNCs) with hyaluronan (HA) hydrogel is beneficial to the ischemic heart in a rat model of myocardial infarction (MI). However, the therapeutic efficacy and safety must be addressed in large animals before moving onto a clinical trial. Therefore, the effect of combined treatment on MI was investigated in pigs. Coronary artery ligation was performed in minipigs to induce MI followed by an intramyocardial injection of normal saline ( n = 7), HA ( n = 7), normal saline with 1 × 108 freshly isolated MNCs ( n = 8), or HA with 1 × 108 MNCs (HA-MNC; n = 7), with a sham-operated group serving as a control ( n = 7). The response of each experimental group was estimated by echocardiography, ventricular catheterization, and histological analysis. Although injection of HA or MNCs slightly elevated left ventricular ejection fraction, the combined HA-MNC injection showed a significant increase in left ventricular ejection fraction, contractility, infarct size, and neovascularization. Importantly, injection of MNCs with HA also promoted MNC retention and MNC differentiation into vascular lineage cells in pigs. Therefore, this study not only provides evidence but also raises the possibility of using a combined HA-MNC injection as a promising therapy for heart repair.
- Published
- 2014
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