1. Endotracheal tube mucus as a source of airway mucus for rheological study.
- Author
-
Markovetz MR, Subramani DB, Kissner WJ, Morrison CB, Garbarine IC, Ghio A, Ramsey KA, Arora H, Kumar P, Nix DB, Kumagai T, Krunkosky TM, Krause DC, Radicioni G, Alexis NE, Kesimer M, Tiemeyer M, Boucher RC, Ehre C, and Hill DB
- Subjects
- Adult, Aged, Aged, 80 and over, Cations, Monovalent, Female, Healthy Volunteers, Humans, Intubation, Intratracheal, Male, Middle Aged, Polysaccharides classification, Polysaccharides isolation & purification, Potassium metabolism, Proteins classification, Proteins isolation & purification, Sodium metabolism, Sputum chemistry, Trachea physiology, Mucus chemistry, Potassium analysis, Rheology methods, Sodium analysis, Trachea chemistry
- Abstract
Muco-obstructive lung diseases (MOLDs), like cystic fibrosis and chronic obstructive pulmonary disease, affect a spectrum of subjects globally. In MOLDs, the airway mucus becomes hyperconcentrated, increasing osmotic and viscoelastic moduli and impairing mucus clearance. MOLD research requires relevant sources of healthy airway mucus for experimental manipulation and analysis. Mucus collected from endotracheal tubes (ETT) may represent such a source with benefits, e.g., in vivo production, over canonical sample types such as sputum or human bronchial epithelial (HBE) mucus. Ionic and biochemical compositions of ETT mucus from healthy human subjects were characterized and a stock of pooled ETT samples generated. Pooled ETT mucus exhibited concentration-dependent rheologic properties that agreed across spatial scales with reported individual ETT samples and HBE mucus. We suggest that the practical benefits compared with other sample types make ETT mucus potentially useful for MOLD research.
- Published
- 2019
- Full Text
- View/download PDF