1. Processing of meiotic DNA double strand breaks requires cyclin-dependent kinase and multiple nucleases
- Author
-
Andrea Citterio, Maria Pia Longhese, Ilaria Guerini, Giovanna Lucchini, Nicola Manfrini, Manfrini, N, Guerini, I, Citterio, A, Lucchini, G, and Longhese, M
- Subjects
Saccharomyces cerevisiae Proteins ,Spo11 ,DNA repair ,DNA damage ,BIO/18 - GENETICA ,Saccharomyces cerevisiae ,DNA and Chromosomes ,Biochemistry ,Prophase ,DNA Breaks, Double-Stranded ,Phosphorylation ,Molecular Biology ,Recombination, Genetic ,Cyclin-dependent kinase 1 ,Endodeoxyribonucleases ,RecQ Helicases ,biology ,Cell Cycle ,fungi ,DNA Helicases ,Helicase ,Cell Biology ,Endonucleases ,Molecular biology ,Cyclin-Dependent Kinases ,Cell biology ,Meiosis ,enzymes and coenzymes (carbohydrates) ,DNA Topoisomerases, Type II ,Exodeoxyribonucleases ,meiosis, DNA double strand breaks, nucleases ,biology.protein ,biological phenomena, cell phenomena, and immunity ,Homologous recombination ,DNA Damage ,Sgs1 - Abstract
Meiotic recombination requires the formation of programmed Spo11-dependent DNA double strand breaks (DSBs). In Saccharomyces cerevisiae, the Sae2 protein and the Mre11-Rad50-Xrs2 complex are necessary to remove the covalently attached Spo11 protein from the DNA ends, which are then resected by so far unknown nucleases. Here, we demonstrate that phosphorylation of Sae2 Ser-267 by cyclin-dependent kinase 1 (Cdk1) is required to initiate meiotic DSB resection by allowing Spo11 removal from DSB ends. This finding suggests that Cdk1 activity is required for the processing of Spo11-induced DSBs, thus providing a mechanism for coordinating DSB resection with progression through meiotic prophase. Furthermore, the helicase Sgs1 and the nucleases Exo1 and Dna2 participate in lengthening the 5′-3′ resection tracts during meiosis by controlling a step subsequent to Spo11 removal.
- Published
- 2010