1. IL-3 promotes the development of experimental autoimmune encephalitis
- Author
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Manuel Rodriguez Gomez, Fabian Hermann, Nicole Goebel, Yvonne Talke, Gabriela Schiechl, Christine Riedhammer, Matthias Mack, Simone Kutzi, Sonja Hellerbrand, Kerstin Renner, Robert Weissert, Dagmar Halbritter, and Hilke Brühl
- Subjects
0301 basic medicine ,Adult ,CD4-Positive T-Lymphocytes ,Male ,Chemokine ,Adoptive cell transfer ,Encephalomyelitis, Autoimmune, Experimental ,Multiple Sclerosis ,Encephalomyelitis ,Myelin oligodendrocyte glycoprotein ,03 medical and health sciences ,Mice ,0302 clinical medicine ,immune system diseases ,medicine ,Animals ,Humans ,Interleukin 3 ,Autoimmune encephalitis ,biology ,business.industry ,Multiple sclerosis ,Experimental autoimmune encephalomyelitis ,Antibodies, Monoclonal ,General Medicine ,medicine.disease ,Adoptive Transfer ,nervous system diseases ,Mice, Inbred C57BL ,030104 developmental biology ,Immunology ,biology.protein ,Female ,Interleukin-3 ,Myelin-Oligodendrocyte Glycoprotein ,Chemokines ,business ,Cell Adhesion Molecules ,030217 neurology & neurosurgery ,Research Article - Abstract
Little is known about the role of IL-3 in multiple sclerosis (MS) in humans and in experimental autoimmune encephalomyelitis (EAE). Using myelin oligodendrocyte glycoprotein (MOG) peptide-induced EAE, we show that CD4+ T cells are the main source of IL-3 and that cerebral IL-3 expression correlates with the influx of T cells into the brain. Blockade of IL-3 with monoclonal antibodies, analysis of IL-3 deficient mice, and adoptive transfer of leukocytes demonstrate that IL-3 plays an important role for development of clinical symptoms of EAE, for migration of leukocytes into the brain, and for cerebral expression of adhesion molecules and chemokines. In contrast, injection of recombinant IL-3 exacerbates EAE symptoms and cerebral inflammation. In patients with relapsing-remitting MS (RRMS), IL-3 expression by T cells is markedly upregulated during episodes of relapse. Our data indicate that IL-3 plays an important role in EAE and may represent a new target for treatment of MS.
- Published
- 2016