13 results on '"Blane, Beth"'
Search Results
2. The Emergence of Successful Streptococcus pyogenes Lineages through Convergent Pathways of Capsule Loss and Recombination Directing High Toxin Expression
- Author
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Turner, Claire E., primary, Holden, Matthew T. G., additional, Blane, Beth, additional, Horner, Carolyne, additional, Peacock, Sharon J., additional, and Sriskandan, Shiranee, additional
- Published
- 2019
- Full Text
- View/download PDF
3. Pilot Evaluation of a Fully Automated Bioinformatics System for Analysis of Methicillin-Resistant Staphylococcus aureus Genomes and Detection of Outbreaks
- Author
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Brown, Nicholas M., primary, Blane, Beth, additional, Raven, Kathy E., additional, Kumar, Narender, additional, Leek, Danielle, additional, Bragin, Eugene, additional, Rhodes, Paul A., additional, Enoch, David A., additional, Thaxter, Rachel, additional, Parkhill, Julian, additional, and Peacock, Sharon J., additional
- Published
- 2019
- Full Text
- View/download PDF
4. Methodology for Whole-Genome Sequencing of Methicillin-Resistant Staphylococcus aureus Isolates in a Routine Hospital Microbiology Laboratory
- Author
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Raven, Kathy E, Blane, Beth, Leek, Danielle, Churcher, Carol, Kokko-Gonzales, Paula, Pugazhendhi, Dhamayanthi, Fraser, Louise, Betley, Jason, Parkhill, Julian, Peacock, Sharon J, Parkhill, Julian [0000-0002-7069-5958], and Apollo - University of Cambridge Repository
- Subjects
Methicillin-Resistant Staphylococcus aureus ,Microbiological Techniques ,clinical application ,Whole Genome Sequencing ,whole-genome sequencing ,Diagnostic Tests, Routine ,Humans ,MRSA ,Staphylococcal Infections ,Laboratories, Hospital ,Genome, Bacterial ,Multilocus Sequence Typing - Abstract
There is growing evidence for the value of bacterial whole-genome sequencing in hospital outbreak investigations. Our aim was to develop methods that support efficient and accurate low-throughput clinical sequencing of methicillin-resistant Staphylococcus aureus (MRSA) isolates. Using a test panel of 25 MRSA isolates previously associated with outbreak investigations, we devised modifications to library preparation that reduced the processing time by 1 hour. We determined the maximum number of isolates that could be sequenced per run using an Illumina MiniSeq platform and a 13-hour (overnight) run time, which equated to 21 MRSA isolates and 3 controls (no template, positive, and negative). Repeatability and reproducibility assays based on this sequencing methodology demonstrated 100% accuracy in assigning species and sequence type (ST) and in detecting mecA Established genetic relatedness between isolates was recapitulated. Quality control (QC) metrics were evaluated over nine sequencing runs. Of the test panel MRSA genomes, 168/173 (97%) passed QC metrics based on the correct species assigned, detection of mecA and ST, and depth/coverage metrics. An evaluation of contamination in these 9 runs showed that positive and negative controls and test MRSA sequence files contained
- Published
- 2019
5. Genomic Surveillance of <named-content content-type='genus-species'>Enterococcus faecium</named-content> Reveals Limited Sharing of Strains and Resistance Genes between Livestock and Humans in the United Kingdom
- Author
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Gouliouris, Theodore, Raven, Kathy E, Ludden, Catherine, Blane, Beth, Corander, Jukka, Horner, Carolyne S, Hernandez-Garcia, Juan, Wood, Paul, Hadjirin, Nazreen F, Radakovic, Milorad, Holmes, Mark A, De Goffau, Marcus, Brown, Nicholas M, Parkhill, Julian, Peacock, Sharon J, Parkhill, Julian [0000-0002-7069-5958], and Apollo - University of Cambridge Repository
- Subjects
vancomycin resistant ,Farms ,Genotype ,Swine ,Enterococcus faecium ,Microbial Sensitivity Tests ,Wastewater ,Polymorphism, Single Nucleotide ,Microbiology ,Vancomycin-Resistant Enterococci ,Clinical Science and Epidemiology ,Drug Resistance, Multiple, Bacterial ,parasitic diseases ,Prevalence ,Animals ,Humans ,One Health ,Gram-Positive Bacterial Infections ,Phylogeny ,Whole Genome Sequencing ,vancomycin-resistant ,Vancomycin Resistance ,United Kingdom ,QR1-502 ,Anti-Bacterial Agents ,Meat Products ,livestock ,genome sequencing ,Cross-Sectional Studies ,Epidemiological Monitoring ,Genome, Bacterial ,Research Article - Abstract
The rise in rates of human infection caused by vancomycin-resistant Enterococcus faecium (VREfm) strains between 1988 to the 2000s in Europe was suggested to be associated with acquisition from livestock. As a result, the European Union banned the use of the glycopeptide drug avoparcin as a growth promoter in livestock feed. While some studies reported a decrease in VREfm in livestock, others reported no reduction. Here, we report the first livestock VREfm prevalence survey in the UK since 2003 and the first large-scale study using whole-genome sequencing to investigate the relationship between E. faecium strains in livestock and humans. We found a low prevalence of VREfm in retail meat and limited evidence for recent sharing of strains between livestock and humans with bloodstream infection. There was evidence for limited sharing of genes encoding antibiotic resistance between these reservoirs, a finding which requires further research., Vancomycin-resistant Enterococcus faecium (VREfm) is a major cause of nosocomial infection and is categorized as high priority by the World Health Organization global priority list of antibiotic-resistant bacteria. In the past, livestock have been proposed as a putative reservoir for drug-resistant E. faecium strains that infect humans, and isolates of the same lineage have been found in both reservoirs. We undertook cross-sectional surveys to isolate E. faecium (including VREfm) from livestock farms, retail meat, and wastewater treatment plants in the United Kingdom. More than 600 isolates from these sources were sequenced, and their relatedness and antibiotic resistance genes were compared with genomes of almost 800 E. faecium isolates from patients with bloodstream infection in the United Kingdom and Ireland. E. faecium was isolated from 28/29 farms; none of these isolates were VREfm, suggesting a decrease in VREfm prevalence since the last UK livestock survey in 2003. However, VREfm was isolated from 1% to 2% of retail meat products and was ubiquitous in wastewater treatment plants. Phylogenetic comparison demonstrated that the majority of human and livestock-related isolates were genetically distinct, although pig isolates from three farms were more genetically related to human isolates from 2001 to 2004 (minimum of 50 single-nucleotide polymorphisms [SNPs]). Analysis of accessory (variable) genes added further evidence for distinct niche adaptation. An analysis of acquired antibiotic resistance genes and their variants revealed limited sharing between humans and livestock. Our findings indicate that the majority of E. faecium strains infecting patients are largely distinct from those from livestock in this setting, with limited sharing of strains and resistance genes.
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- 2018
6. One Health Genomic Surveillance of Escherichia coli Demonstrates Distinct Lineages and Mobile Genetic Elements in Isolates from Humans versus Livestock
- Author
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Ludden, Catherine, primary, Raven, Kathy E., additional, Jamrozy, Dorota, additional, Gouliouris, Theodore, additional, Blane, Beth, additional, Coll, Francesc, additional, de Goffau, Marcus, additional, Naydenova, Plamena, additional, Horner, Carolyne, additional, Hernandez-Garcia, Juan, additional, Wood, Paul, additional, Hadjirin, Nazreen, additional, Radakovic, Milorad, additional, Brown, Nicholas M., additional, Holmes, Mark, additional, Parkhill, Julian, additional, and Peacock, Sharon J., additional
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- 2019
- Full Text
- View/download PDF
7. Genomic Surveillance of Enterococcus faecium Reveals Limited Sharing of Strains and Resistance Genes between Livestock and Humans in the United Kingdom
- Author
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Gouliouris, Theodore, primary, Raven, Kathy E., additional, Ludden, Catherine, additional, Blane, Beth, additional, Corander, Jukka, additional, Horner, Carolyne S., additional, Hernandez-Garcia, Juan, additional, Wood, Paul, additional, Hadjirin, Nazreen F., additional, Radakovic, Milorad, additional, Holmes, Mark A., additional, de Goffau, Marcus, additional, Brown, Nicholas M., additional, Parkhill, Julian, additional, and Peacock, Sharon J., additional
- Published
- 2018
- Full Text
- View/download PDF
8. Evolution of the Staphylococcus argenteus ST2250 Clone in Northeastern Thailand Is Linked with the Acquisition of Livestock-Associated Staphylococcal Genes
- Author
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Moradigaravand, Danesh, primary, Jamrozy, Dorota, additional, Mostowy, Rafal, additional, Anderson, Annaliesa, additional, Nickerson, Emma K., additional, Thaipadungpanit, Janjira, additional, Wuthiekanun, Vanaporn, additional, Limmathurotsakul, Direk, additional, Tandhavanant, Sarunporn, additional, Wikraiphat, Chanthiwa, additional, Wongsuvan, Gumphol, additional, Teerawattanasook, Nittaya, additional, Jutrakul, Yaowaruk, additional, Srisurat, Nuttiya, additional, Chaimanee, Prajuab, additional, Eoin West, T., additional, Blane, Beth, additional, Parkhill, Julian, additional, Chantratita, Narisara, additional, and Peacock, Sharon J., additional
- Published
- 2017
- Full Text
- View/download PDF
9. One Health Genomic Surveillance of Escherichia coli Demonstrates Distinct Lineages and Mobile Genetic Elements in Isolates from Humans versus Livestock
- Author
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Ludden, Catherine, Raven, Kathy E, Jamrozy, Dorota, Gouliouris, Theodore, Blane, Beth, Coll, Francesc, De Goffau, Marcus, Naydenova, Plamena, Horner, Carolyne, Hernandez-Garcia, Juan, Wood, Paul, Hadjirin, Nazreen, Radakovic, Milorad, Brown, Nicholas M, Holmes, Mark, Parkhill, Julian, and Peacock, Sharon J
- Subjects
2. Zero hunger ,Livestock ,Meat ,Whole Genome Sequencing ,Computational Biology ,Genetic Variation ,Genomics ,Sequence Analysis, DNA ,3. Good health ,Interspersed Repetitive Sequences ,Cross-Sectional Studies ,ESBL ,England ,Genes, Bacterial ,Drug Resistance, Bacterial ,Epidemiological Monitoring ,Escherichia coli ,Prevalence ,Animals ,Humans ,antimicrobial resistance ,One Health ,Escherichia coli Infections - Abstract
Livestock have been proposed as a reservoir for drug-resistant Escherichia coli that infect humans. We isolated and sequenced 431 E. coli isolates (including 155 extended-spectrum β-lactamase [ESBL]-producing isolates) from cross-sectional surveys of livestock farms and retail meat in the East of England. These were compared with the genomes of 1,517 E. coli bacteria associated with bloodstream infection in the United Kingdom. Phylogenetic core genome comparisons demonstrated that livestock and patient isolates were genetically distinct, suggesting that E. coli causing serious human infection had not directly originated from livestock. In contrast, we observed highly related isolates from the same animal species on different farms. Screening all 1,948 isolates for accessory genes encoding antibiotic resistance revealed 41 different genes present in variable proportions in human and livestock isolates. Overall, we identified a low prevalence of shared antimicrobial resistance genes between livestock and humans based on analysis of mobile genetic elements and long-read sequencing. We conclude that within the confines of our sampling framework, there was limited evidence that antimicrobial-resistant pathogens associated with serious human infection had originated from livestock in our region.IMPORTANCE The increasing prevalence of E. coli bloodstream infections is a serious public health problem. We used genomic epidemiology in a One Health study conducted in the East of England to examine putative sources of E. coli associated with serious human disease. E. coli from 1,517 patients with bloodstream infections were compared with 431 isolates from livestock farms and meat. Livestock-associated and bloodstream isolates were genetically distinct populations based on core genome and accessory genome analyses. Identical antimicrobial resistance genes were found in livestock and human isolates, but there was limited overlap in the mobile elements carrying these genes. Within the limitations of sampling, our findings do not support the idea that E. coli causing invasive disease or their resistance genes are commonly acquired from livestock in our region.
10. The Emergence of Successful Streptococcus pyogenes Lineages through Convergent Pathways of Capsule Loss and Recombination Directing High Toxin Expression
- Author
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Turner, Claire E, Holden, Matthew TG, Blane, Beth, Horner, Carolyne, Peacock, Sharon J, and Sriskandan, Shiranee
- Subjects
Antigens, Bacterial ,Base Sequence ,Genotype ,Streptococcus pyogenes ,Virulence Factors ,group A Streptococcus ,homologous recombination ,NADase ,3. Good health ,NAD+ Nucleosidase ,Bacterial Proteins ,England ,hyaluronic acid capsule ,whole-genome sequencing ,Streptococcal Infections ,Streptolysins ,Humans ,convergent evolution ,streptolysin O ,Bacterial Capsules ,Bacterial Outer Membrane Proteins ,Toxins, Biological - Abstract
Gene transfer and homologous recombination in Streptococcus pyogenes has the potential to trigger the emergence of pandemic lineages, as exemplified by lineages of emm1 and emm89 that emerged in the 1980s and 2000s, respectively. Although near-identical replacement gene transfer events in the nga (NADase) and slo (streptolysin O) loci conferring high expression of these toxins underpinned the success of these lineages, extension to other emm genotype lineages is unreported. The emergent emm89 lineage was characterized by five regions of homologous recombination additional to nga-slo, including complete loss of the hyaluronic acid capsule synthesis locus hasABC, a genetic trait replicated in two other leading emm types and recapitulated by other emm types by inactivating mutations. We hypothesized that other leading genotypes may have undergone similar recombination events. We analyzed a longitudinal data set of genomes from 344 clinical invasive disease isolates representative of locations across England, dating from 2001 to 2011, and an international collection of S. pyogenes genomes representing 54 different genotypes and found frequent evidence of recombination events at the nga-slo locus predicted to confer higher toxin genotype. We identified multiple associations between recombination at this locus and inactivating mutations within hasAB, suggesting convergent evolutionary pathways in successful genotypes. This included common genotypes emm28 and emm87. The combination of no or low capsule and high expression of nga and slo may underpin the success of many emergent S. pyogenes lineages of different genotypes, triggering new pandemics, and could change the way S. pyogenes causes disease.IMPORTANCEStreptococcus pyogenes is a genetically diverse pathogen, with over 200 different genotypes defined by emm typing, but only a minority of these genotypes are responsible for the majority of human infection in high-income countries. Two prevalent genotypes associated with disease rose to international dominance following recombination of a toxin locus that conferred increased expression. Here, we found that recombination of this locus and promoter has occurred in other diverse genotypes, events that may allow these genotypes to expand in the population. We identified an association between the loss of hyaluronic acid capsule synthesis and high toxin expression, which we propose may be associated with an adaptive advantage. As S. pyogenes pathogenesis depends both on capsule and toxin production, new variants with altered expression may result in abrupt changes in the molecular epidemiology of this pathogen in the human population over time.
11. Pilot Evaluation of a Fully Automated Bioinformatics System for Analysis of Methicillin-Resistant Staphylococcus aureus Genomes and Detection of Outbreaks
- Author
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Brown, Nicholas M, Blane, Beth, Raven, Kathy E, Kumar, Narender, Leek, Danielle, Bragin, Eugene, Rhodes, Paul A, Enoch, David A, Thaxter, Rachel, Parkhill, Julian, and Peacock, Sharon J
- Subjects
Automation, Laboratory ,Methicillin-Resistant Staphylococcus aureus ,Staphylococcus aureus ,Whole Genome Sequencing ,microbiology ,Computational Biology ,Pilot Projects ,bioinformatics ,Sequence Analysis, DNA ,Staphylococcal Infections ,3. Good health ,Anti-Bacterial Agents ,Bacterial Typing Techniques ,Disease Outbreaks ,Methicillin ,England ,whole-genome sequencing ,Humans ,Prospective Studies ,Genome, Bacterial ,Multilocus Sequence Typing - Abstract
Genomic surveillance that combines bacterial sequencing and epidemiological information will become the gold standard for outbreak detection, but its clinical translation is hampered by the lack of automated interpretation tools. We performed a prospective pilot study to evaluate the analysis of methicillin-resistant Staphylococcus aureus (MRSA) genomes using the Next Gen Diagnostics (NGD) automated bioinformatics system. Seventeen unselected MRSA-positive patients were identified in a clinical microbiology laboratory in England over a period of 2 weeks in 2018, and 1 MRSA isolate per case was sequenced on the Illumina MiniSeq instrument. The NGD system automatically activated after sequencing and processed fastq folders to determine species, multilocus sequence type, the presence of a mec gene, antibiotic susceptibility predictions, and genetic relatedness based on mapping to a reference MRSA genome and detection of pairwise core genome single-nucleotide polymorphisms. The NGD system required 90 s per sample to automatically analyze data from each run, the results of which were automatically displayed. The same data were independently analyzed using a research-based approach. There was full concordance between the two analysis methods regarding species (S. aureus), detection of mecA, sequence type assignment, and detection of genetic determinants of resistance. Both analysis methods identified two MRSA clusters based on relatedness, one of which contained 3 cases that were involved in an outbreak linked to a clinic and ward associated with diabetic patient care. We conclude that, in this pilot study, the NGD system provided rapid and accurate data that could support infection control practices.
12. Methodology for Whole-Genome Sequencing of Methicillin-Resistant Staphylococcus aureus Isolates in a Routine Hospital Microbiology Laboratory
- Author
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Raven, Kathy E, Blane, Beth, Leek, Danielle, Churcher, Carol, Kokko-Gonzales, Paula, Pugazhendhi, Dhamayanthi, Fraser, Louise, Betley, Jason, Parkhill, Julian, and Peacock, Sharon J
- Subjects
Methicillin-Resistant Staphylococcus aureus ,Microbiological Techniques ,clinical application ,Whole Genome Sequencing ,whole-genome sequencing ,Diagnostic Tests, Routine ,Humans ,MRSA ,Staphylococcal Infections ,Laboratories, Hospital ,Genome, Bacterial ,3. Good health ,Multilocus Sequence Typing - Abstract
There is growing evidence for the value of bacterial whole-genome sequencing in hospital outbreak investigations. Our aim was to develop methods that support efficient and accurate low-throughput clinical sequencing of methicillin-resistant Staphylococcus aureus (MRSA) isolates. Using a test panel of 25 MRSA isolates previously associated with outbreak investigations, we devised modifications to library preparation that reduced the processing time by 1 hour. We determined the maximum number of isolates that could be sequenced per run using an Illumina MiniSeq platform and a 13-hour (overnight) run time, which equated to 21 MRSA isolates and 3 controls (no template, positive, and negative). Repeatability and reproducibility assays based on this sequencing methodology demonstrated 100% accuracy in assigning species and sequence type (ST) and in detecting mecA Established genetic relatedness between isolates was recapitulated. Quality control (QC) metrics were evaluated over nine sequencing runs. Of the test panel MRSA genomes, 168/173 (97%) passed QC metrics based on the correct species assigned, detection of mecA and ST, and depth/coverage metrics. An evaluation of contamination in these 9 runs showed that positive and negative controls and test MRSA sequence files contained
13. Methodology for Whole-Genome Sequencing of Methicillin-Resistant Staphylococcus aureus Isolates in a Routine Hospital Microbiology Laboratory.
- Author
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Raven KE, Blane B, Leek D, Churcher C, Kokko-Gonzales P, Pugazhendhi D, Fraser L, Betley J, Parkhill J, and Peacock SJ
- Subjects
- Diagnostic Tests, Routine, Humans, Laboratories, Hospital, Methicillin-Resistant Staphylococcus aureus isolation & purification, Microbiological Techniques, Multilocus Sequence Typing, Genome, Bacterial, Methicillin-Resistant Staphylococcus aureus genetics, Staphylococcal Infections diagnosis, Staphylococcal Infections microbiology, Whole Genome Sequencing methods
- Abstract
There is growing evidence for the value of bacterial whole-genome sequencing in hospital outbreak investigations. Our aim was to develop methods that support efficient and accurate low-throughput clinical sequencing of methicillin-resistant Staphylococcus aureus (MRSA) isolates. Using a test panel of 25 MRSA isolates previously associated with outbreak investigations, we devised modifications to library preparation that reduced the processing time by 1 hour. We determined the maximum number of isolates that could be sequenced per run using an Illumina MiniSeq platform and a 13-hour (overnight) run time, which equated to 21 MRSA isolates and 3 controls (no template, positive, and negative). Repeatability and reproducibility assays based on this sequencing methodology demonstrated 100% accuracy in assigning species and sequence type (ST) and in detecting mecA Established genetic relatedness between isolates was recapitulated. Quality control (QC) metrics were evaluated over nine sequencing runs. Of the test panel MRSA genomes, 168/173 (97%) passed QC metrics based on the correct species assigned, detection of mecA and ST, and depth/coverage metrics. An evaluation of contamination in these 9 runs showed that positive and negative controls and test MRSA sequence files contained <0.14% and <0.48% of fragments that matched another species, respectively. Deliberate contamination experiments confirmed that this was insufficient to impact data interpretation. These methods support reliable and reproducible clinical MRSA sequencing with a turnaround time (from DNA extraction to availability of data files) of 24 hours., (Copyright © 2019 Raven et al.)
- Published
- 2019
- Full Text
- View/download PDF
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