1. Structural Map of a MicroRNA-122:Hepatitis C Virus Complex
- Author
-
Shripa Patel, Michael Eckart, Jeffrey S. Glenn, Edward A. Pham, Menashe Elazar, and Phillip S. Pang
- Subjects
Untranslated region ,Hepatitis C virus ,Hepacivirus ,Immunology ,Molecular Sequence Data ,medicine.disease_cause ,Virus Replication ,Microbiology ,Primer extension ,Nucleic acid secondary structure ,Virology ,medicine ,Humans ,Genetics ,biology ,Base Sequence ,RNA ,biology.organism_classification ,Hepatitis C ,Virus-Cell Interactions ,NS2-3 protease ,MicroRNAs ,Viral replication ,Insect Science ,Nucleic Acid Conformation ,RNA, Viral ,5' Untranslated Regions - Abstract
MicroRNA-122 (miR-122) enhances hepatitis C virus (HCV) fitness via targeting two sites in the 5′-untranslated region (UTR) of HCV. We used selective 2′-hydroxyl acylation analyzed by primer extension to resolve the HCV 5′-UTR's RNA secondary structure in the presence of miR-122. Nearly all nucleotides in miR-122 are involved in targeting the second site, beyond classic seed base pairings. These additional interactions enhance HCV replication in cell culture. To our knowledge, this is the first biophysical study of this complex to reveal the importance of ‘tail’ miR-122 nucleotide interactions.
- Published
- 2012