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Your search keyword '"Greenhill, Andrew"' showing total 11 results
Start Over Author "Greenhill, Andrew" Publisher american society for microbiology
11 results on '"Greenhill, Andrew"'

3. Impact of Intermittent Preventive Treatment in Pregnancy with Azithromycin-Containing Regimens on Maternal Nasopharyngeal Carriage and Antibiotic Sensitivity of Streptococcus pneumoniae, Haemophilus influenzae, and Staphylococcus aureus: a Cross-Sectional Survey at Delivery

Author

Unger, Holger W., primary, Aho, Celestine, additional, Ome-Kaius, Maria, additional, Wangnapi, Regina A., additional, Umbers, Alexandra J., additional, Jack, Wanda, additional, Lafana, Alice, additional, Michael, Audrey, additional, Hanieh, Sarah, additional, Siba, Peter, additional, Mueller, Ivo, additional, Greenhill, Andrew R., additional, and Rogerson, Stephen J., additional

Published
2015
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4. Multilocus sequence typing of Streptococcus pneumoniae by use of mass spectrometry

Author

Dunne, Eileen M., Ong, Eng Kok, Moser, Ralp J., Siba, Peter M., Phuanukoonnon, Suparat, Greenhill, Andrew R., Robins-Browne, Roy M., Mulholland, E. Kim, Satzke, Catherine, Dunne, Eileen M., Ong, Eng Kok, Moser, Ralp J., Siba, Peter M., Phuanukoonnon, Suparat, Greenhill, Andrew R., Robins-Browne, Roy M., Mulholland, E. Kim, and Satzke, Catherine

Abstract

Multilocus sequence typing (MLST) is an important tool for the global surveillance of bacterial pathogens that is performed by comparing the sequences of designated housekeeping genes. We developed and tested a novel mass spectrometry-based method for MLST of Streptococcus pneumoniae. PCR amplicons were subjected to in vitro transcription and base-specific cleavage, followed by analysis of the resultant fragments by using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). Comparison of the cleavage fragment peak patterns to a reference sequence set permitted automated identification of alleles. Validation experiments using 29 isolates of S. pneumoniae revealed that the results of MALDI-TOF MS MLST matched those obtained by traditional sequence-based MLST for 99% of alleles and that the MALDI-TOF MS method accurately identified two single-nucleotide variations. The MADLI-TOF MS method was then used for MLST analysis of 43 S. pneumoniae isolates from Papua New Guinean children. The majority of the isolates present in this population were not clonal and contained seven new alleles and 30 previously unreported sequence types.

Published
2011

11. Detection and characterisation of Salmonella enterica serovar Hvittingfoss in Bar-tailed Godwits (Limosa lapponica) from Roebuck Bay, north-western Australia.

Author

Smith, Hannah G., Bean, David C., Hawkey, Jane, Clarke, Rohan H., Loyn, Richard, Larkins, Jo-Ann, Hassell, Chris, Valcanis, Mary, Pitchers, William, and Greenhill, Andrew R.

Subjects
*SALMONELLA enterica, *SALMONELLA detection, *ANIMAL populations, *BACTERIAL cultures, *SALMONELLA, *BIRDS
Abstract

Salmonella enterica serovar Hvittingfoss is an important foodborne serotype of Salmonella, being detected in many countries where surveillance is conducted. Outbreaks can occur, and there was a recent multistate foodborne outbreak in Australia. S. Hvittingfoss can be found in animal populations, though a definitive animal host has not been established. Six species of birds were sampled at Roebuck Bay, a designated Ramsar site in northwestern Australia, resulting in 326 cloacal swabs for bacterial culture. Among a single flock of 63 bar-tailed godwits (Limosa lapponica menzbieri) caught at Wader Spit, Roebuck Bay, in 2018, 17 (27%) were culture positive for Salmonella. All other birds were negative for Salmonella. The isolates were identified as Salmonella enterica serovar Hvittingfoss. Phylogenetic analysis revealed a close relationship between isolates collected from godwits and the S. Hvittingfoss strain responsible for a 2016 multistate foodborne outbreak originating from tainted cantaloupes (rock melons) in Australia. While it is not possible to determine how this strain of S. Hvittingfoss was introduced into the bar-tailed godwits, these findings show that wild Australian birds are capable of carrying Salmonella strains of public health importance. [ABSTRACT FROM AUTHOR]

Published
2020
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