8 results on '"Mohammad Javad Najafzadeh"'
Search Results
2. Epidemiology and Antifungal Susceptibility of Candida Species Isolated from 10 Tertiary Care Hospitals in Iran
- Author
-
Parisa Badiee, Teun Boekhout, Pardis Haddadi, Rasoul Mohammadi, Abdolkarim Ghadimi-Moghadam, Jafar Soltani, Ali Zarei Mahmoudabadi, Seyyed Amin Ayatollahi Mousavi, Mohammad Javad Najafzadeh, Kambiz Diba, Ali Reza Salimi-Khorashad, Maneli Amin Shahidi, Fatemeh Ghasemi, and Hadis Jafarian
- Subjects
Candida species ,caspofungin ,voriconazole ,amphotericin B ,isavuconazole ,itraconazole ,Microbiology ,QR1-502 - Abstract
ABSTRACT In recent decades, the incidence of Candida infections has increased in immunocompromised patients. This multicenter study aimed to evaluate in vitro antifungal activities of 8 antifungal agents against the Candida species isolated from 10 university hospitals in Iran. During the period from Dec 2019 to Dec 2021, Candida species were collected from clinical samples of patients. The isolates were identified by PCR restriction fragment length polymorphism and sequencing methods. The antifungal susceptibility tests of each isolate to eight antifungal agents were performed according to the microdilution CLSI M27, M59, and M60 standard methods. A total of 598 Candida strains were isolated from clinical samples. The most commonly isolated Candida species was C. albicans, followed by C. glabrata, C. parapsilosis, Debaryomyces hansenii (Candida famata), C. tropicalis, Pichia kudriavzevii (Candida krusei), C. orthopsilosis, Meyerozyma guilliermondii (Candida guilliermondii), Kluyveromyces marxianus (Candida kefyr), and Clavispora lusitaniae (Candida lusitaniae). MIC90 values in all Candida species were as follows: 0.25 μg/mL for caspofungin and voriconazole; 0.5 μg/mL for amphotericin B and isavuconazole; 2 μg/mL for itraconazole, luliconazole, and posaconazole; and 16 μg/mL for fluconazole. Although 30/285 C. albicans, 15/31 C. hansenii, 3/12 M. guilliermondii, 67/125 C. glabrata, 5/15 P. kudriavzevii, 6/60 C. parapsilosis, and 5/23 C. tropicalis isolates were multiazole resistant with resistance to 2 to 4 azoles, pan-azole resistance was not observed. According to our data, Candida albicans and C. glabrata were the most frequent species isolated from clinical samples in Iran. Caspofungin and voriconazole, with lower MIC90 values, are the most effective than other antifungal agents for the treatment of Candida infections in this region. IMPORTANCE Candida species cause severe invasive infections of the heart, brain, eyes, bones, and other parts of the body. Knowledge of regional distributions of causative Candida agents and their antifungal susceptibility patterns can help to monitor resistance to antifungal agents of various species and support local and national surveillance programs. In the present study, C. albicans and C. glabrata were the most frequently isolated species from clinical samples in Iran. Increasing rates of non-albicans Candida isolates from the Iranian population should be looked at as alarming due to various levels of intrinsic MIC values or resistance to various antifungal drugs. Caspofungin and voriconazole are recommended over fluconazole for the treatment of Candida infections in the study region. However, amphotericin B and isavuconazole are also active against the most common Candida species isolated from patients. Pan azole-resistant Candida species were not observed in the present study.
- Published
- 2022
- Full Text
- View/download PDF
3. Multicenter Study of Susceptibility of Aspergillus Species Isolated from Iranian University Hospitals to Seven Antifungal Agents
- Author
-
Parisa Badiee, Teun Boekhout, Ali Zarei Mahmoudabadi, Rasoul Mohammadi, Seyyed Amin Ayatollahi Mousavi, Mohammad Javad Najafzadeh, Jafar Soltani, Jamal Hashemi, Kambiz Diba, Abdolkarim Ghadimi-Moghadam, Ali Reza Salimi-Khorashad, Tahereh Shokohi, Maneli Amin Shahidi, Fatemeh Ghasemi, and Hadis Jafarian
- Subjects
amphotericin B ,antifungal susceptibility ,Aspergillus ,azoles ,echinocandins ,Iran ,Microbiology ,QR1-502 - Abstract
ABSTRACT Aspergillus species are a major cause of life-threatening invasive infections and noninvasive diseases. This study seeks to investigate the frequency of Aspergillus species among Iranian patients and their susceptibility to seven antifungals. In a cross-sectional study, 233 Aspergillus isolates were collected from 11 university hospitals in Iran between 2018 and 2021. Aspergillus isolates were identified based on colony morphology, microscopic characteristics, PCR-restriction fragment length polymorphism (RFLP), and sequencing of the beta-tubulin gene. The CLSI M38-A2 reference methodology was used for antifungal susceptibility testing of amphotericin B, voriconazole, posaconazole, itraconazole, luliconazole, isavuconazole, and caspofungin. Members of Aspergillus section Flavi (117/233, 50.2%), Aspergillus section Nigri (77/233, 33.1%), Aspergillus section Fumigati (21/233, 9%), Aspergillus section Terrei (14/233, 6%), Aspergillus pseudodeflectus (2/233, 0.85%), and Aspergillus melleus (2/233, 0.85%) were isolated from the samples. The lowest 0.25 MIC90 values for all isolates tested were for luliconazole (0.016 μg/mL) and isavuconazole (0.250 μg/mL), and the highest value was observed for itraconazole (≥ 8μg/mL). The 90% minimum effective concentration (MEC90) value for caspofungin was 0.125 μg/mL. MIC90 values for voriconazole, amphotericin B, and posaconazole were 1, 2, and 2 μg/mL, respectively. The non-wild-type species were presented for amphotericin B (3%), voriconazole (1.3%), posaconazole (2.6%), luliconazole (1.3%), isavuconazole (1.7%), and caspofungin (4.7%). Positive correlations in the MIC values of azole antifungals were observed, and using one azole increases the MIC value rates of other ones. None of the species were pan-azole resistant. Species of Aspergillus section Flavi were the most common Aspergillus species isolated from Iranian samples. Luliconazole, caspofungin, and isavuconazole present the most effective antifungal agents for treatment of infection due to Aspergillus species. Susceptibility tests should be performed frequently in each region for the best management of patients. IMPORTANCE Aspergillus species are the leading cause of invasive aspergillosis in immunocompromised hosts. The susceptibility of Aspergillus species to antifungal agents might be different. Azole-resistant species have emerged worldwide. Performing susceptibility testing in each region can help in the best management of patients. Here, we show the epidemiology and distribution of Aspergillus species in Iran and their susceptibility patterns for seven antifungal agents. The significant points of the present study are that species of Aspergillus section Flavi are the most prevalent Aspergillus species isolated from 11 university hospitals. Luliconazole, caspofungin, and isavuconazole were effective antifungal agents against all Aspergillus species.
- Published
- 2022
- Full Text
- View/download PDF
4. Discovery of New Trichophyton Members, T. persicum and T. spiraliforme spp. nov., as a Cause of Highly Inflammatory Tinea Cases in Iran and Czechia
- Author
-
Adéla Čmoková, Ali Rezaei-Matehkolaei, Ivana Kuklová, Miroslav Kolařík, Forough Shamsizadeh, Saham Ansari, Maral Gharaghani, Viera Miňovská, Mohammad Javad Najafzadeh, Sadegh Nouripour‐Sisakht, Takashi Yaguchi, Kamiar Zomorodian, Hossein Zarrinfar, and Vit Hubka
- Subjects
dermatophytosis ,molecular epidemiology ,multigene phylogeny ,skin mycoses ,Trichophyton benhamiae complex ,zoonotic infections ,Microbiology ,QR1-502 - Abstract
ABSTRACT Pathogens from the Trichophyton benhamiae complex are one of the most important causes of animal mycoses with significant zoonotic potential. In light of the recently revised taxonomy of this complex, we retrospectively identified 38 Trichophyton isolates that could not be resolved into any of the existing species. These strains were isolated from Iranian and Czech patients during molecular epidemiological surveys on dermatophytosis and were predominantly associated with highly inflammatory tinea corporis cases, suggesting possible zoonotic etiology. Subsequent phylogenetic (4 markers), population genetic (10 markers), and phenotypic analyses supported recognition of two novel species. The first species, Trichophyton persicum sp. nov., was identified in 36 cases of human dermatophytosis and one case of feline dermatophytosis, mainly in Southern and Western Iran. The second species, Trichophyton spiraliforme sp. nov., is only known from a single case of tinea corporis in a Czech patient who probably contracted the infection from a dog. Although the zoonotic sources of infections summarized in this study are very likely, little is known about the host spectrum of these pathogens. Awareness of these new pathogens among clinicians should refine our knowledge about their poorly explored geographic distribution. IMPORTANCE In this study, we describe two novel agents of dermatophytosis and summarize the clinical manifestation of infections. These new pathogens were discovered thanks to long-term molecular epidemiological studies conducted in Czechia and Iran. Zoonotic origins of the human infections are highly probable, but the animal hosts of these pathogens are poorly known. Further research is needed to refine our knowledge about these new dermatophytes.
- Published
- 2021
- Full Text
- View/download PDF
5. Three Isothermal Amplification Techniques for Rapid Identification of Cladophialophora carrionii, an Agent of Human Chromoblastomycosis
- Author
-
G. Sybren de Hoog, Jiufeng Sun, Ruoyu Li, A. H. G. Gerrits van den Ende, Weihua Pan, Shuwen Deng, Mohammad Javad Najafzadeh, Min Chen, L. Yang, and Wanqing Liao
- Subjects
Microbiology (medical) ,Time Factors ,Molecular Sequence Data ,Loop-mediated isothermal amplification ,Mycology ,Biology ,Sensitivity and Specificity ,chemistry.chemical_compound ,Peptide Elongation Factor 1 ,Ascomycota ,DNA, Ribosomal Spacer ,medicine ,Humans ,Multiplex ,Multiplex ligation-dependent probe amplification ,Internal transcribed spacer ,DNA, Fungal ,Gene ,DNA Primers ,Chromoblastomycosis ,Sequence Analysis, DNA ,Nucleic acid amplification technique ,medicine.disease ,Molecular biology ,Molecular Diagnostic Techniques ,chemistry ,Oligonucleotide Probes ,Nucleic Acid Amplification Techniques ,DNA - Abstract
In this study, we developed rapid and sensitive assays for the detection of Cladophialophora carrionii , a common agent of human chromoblastomycosis. The isothermal techniques evaluated were rolling-circle amplification (RCA), multiplex ligation-dependent probe amplification (MLPA), and loop-mediated isothermal amplification (LAMP). The probes for RCA and MLPA were designed with target sequences in the rDNA internal transcribed spacer gene ( ITS ) region, and LAMP primers were designed using the elongation factor 1α gene ( EF1 ); these probes and primers specifically amplified DNA of isolates of the species. The three techniques were sufficiently specific and sensitive for discriminating target DNA of C. carrionii from that of related Cladophialophora species and other agents of chromoblastomycosis. RCA, MLPA, and LAMP are advantageous in their reliability and ease of operation compared to standard PCR and conventional methods.
- Published
- 2014
- Full Text
- View/download PDF
6. In Vitro Activities of Eight Antifungal Drugs against 106 Waterborne and Cutaneous Exophiala Species
- Author
-
Jacques F. Meis, Ilse Curfs-Breuker, Vânia Aparecida Vicente, S.A. Shamsian, Ali Rezaei-Matehkolaei, M. Saradeghi Keisari, Peiying Feng, G.S. de Hoog, and Mohammad Javad Najafzadeh
- Subjects
Posaconazole ,Antifungal Agents ,Pyridines ,Itraconazole ,Microbial Sensitivity Tests ,Biology ,Pharmacology ,Microbiology ,Echinocandins ,Lipopeptides ,chemistry.chemical_compound ,Caspofungin ,Exophiala ,Amphotericin B ,Nitriles ,medicine ,Pharmacology (medical) ,Fluconazole ,Voriconazole ,Micafungin ,Pathogenesis and modulation of inflammation Infection and autoimmunity [N4i 1] ,Triazoles ,biology.organism_classification ,Pyrimidines ,Infectious Diseases ,chemistry ,Susceptibility ,medicine.drug - Abstract
The in vitro activities of eight antifungal drugs against 106 clinical and environmental isolates of waterborne and cutaneous Exophiala species were tested. The MICs and minimum effective concentrations for 90% of the strains tested ( n = 106) were, in increasing order, as follows: posaconazole, 0.063 μg/ml; itraconazole, 0.25 μg/ml; micafungin, 1 μg/ml; voriconazole, 2 μg/ml; isavuconazole, 4 μg/ml; caspofungin, 8 μg/ml; amphotericin B, 16 μg/ml; fluconazole, 64 μg/ml.
- Published
- 2013
- Full Text
- View/download PDF
7. Species-Specific Antifungal Susceptibility Patterns of Scedosporium and Pseudallescheria Species
- Author
-
G. Sybren de Hoog, Paul E. Verweij, Mohammad Javad Najafzadeh, Corné H. W. Klaassen, Ilse Curfs-Breuker, Jacques F. Meis, Michaela Lackner, and Evolutionary Biology (IBED, FNWI)
- Subjects
Posaconazole ,Antifungal Agents ,Scedosporium boydii ,Microbial Sensitivity Tests ,Biology ,Pharmacology ,Invasive mycoses and compromised host Infection and autoimmunity [N4i 2] ,Microbiology ,Pseudallescheria ,Echinocandins ,Lipopeptides ,chemistry.chemical_compound ,Species Specificity ,Drug Resistance, Fungal ,medicine ,Humans ,Pharmacology (medical) ,Scedosporium ,Amplified Fragment Length Polymorphism Analysis ,Voriconazole ,Dose-Response Relationship, Drug ,Micafungin ,Triazoles ,biology.organism_classification ,bacterial infections and mycoses ,Bacterial Typing Techniques ,Pseudallescheria boydii ,Pyrimidines ,Infectious Diseases ,chemistry ,Susceptibility ,Anidulafungin ,Caspofungin ,medicine.drug - Abstract
Since the separation of Pseudallescheria boydii and P. apiosperma in 2010, limited data on species-specific susceptibility patterns of these and other species of Pseudallescheria and its anamorph Scedosporium have been reported. This study presents the antifungal susceptibility patterns of members affiliated with both entities. Clinical and environmental isolates ( n = 332) from a wide range of sources and origins were identified down to species level and tested according to CLSI M38-A2 against eight antifungal compounds. Whereas P. apiosperma (geometric mean MIC/minimal effective concentration [MEC] values of 0.9, 2.4, 7.4, 16.2, 0.2, 0.8, 1.5, and 6.8 μg/ml for voriconazole, posaconazole, isavuconazole, itraconazole, micafungin, anidulafungin, caspofungin, and amphotericin B, respectively) and P. boydii (geometric mean MIC/MEC values of 0.7, 1.3, 5.7, 13.8, 0.5, 1.4, 2.3, and 11.8 μg/ml for voriconazole, posaconazole, isavuconazole, itraconazole, micafungin, anidulafungin, caspofungin, and amphotericin B, respectively) had similar susceptibility patterns, those for S. aurantiacum , S. prolificans , and S. dehoogii were different from each other. Voriconazole was the only drug with significant activity against S. aurantiacum isolates. The MIC distributions of all drugs except voriconazole did not show a normal distribution and often showed two subpopulations, making a species-based prediction of antifungal susceptibility difficult. Therefore, antifungal susceptibility testing of all clinical isolates remains essential for targeted antifungal therapy. Voriconazole was the only compound with low MIC values (MIC 90 of ≤2 μg/ml) for P. apiosperma and P. boydii . Micafungin and posaconazole showed moderate activity against the majority of Scedosporium strains.
- Published
- 2012
- Full Text
- View/download PDF
8. Rapid identification of Pseudallescheria and Scedosporium strains using Rolling Circle Amplification
- Author
-
Michaela Lackner, Jiufeng Sun, G. Sybren de Hoog, Qiaoyun Lu, Mohammad Javad Najafzadeh, and Evolutionary Biology (IBED, FNWI)
- Subjects
Mycology ,Applied Microbiology and Biotechnology ,Sensitivity and Specificity ,Microbiology ,Scedosporium ,Pseudallescheria ,Limit of Detection ,Internal transcribed spacer ,DNA, Fungal ,Mycological Typing Techniques ,Phylogeny ,Ecology ,biology ,Fungal genetics ,Nucleic acid amplification technique ,biology.organism_classification ,Pseudallescheria boydii ,Rolling circle replication ,DNA, Intergenic ,Restriction fragment length polymorphism ,DNA Probes ,Nucleic Acid Amplification Techniques ,Food Science ,Biotechnology - Abstract
The Pseudallescheria boydii complex, comprising environmental pathogens with Scedosporium anamorphs, has recently been subdivided into five main species: Scedosporium dehoogii , S. aurantiacum , Pseudallescheria minutispora , P. apiosperma , and P. boydii , while the validity of some other taxa is being debated. Several Pseudallescheria and Scedosporium species are indicator organisms of pollution in soil and water. Scedosporium dehoogii in particular is enriched in soils contaminated by aliphatic hydrocarbons. In addition, the fungi may cause life-threatening infections involving the central nervous system in severely impaired patients. For screening purposes, rapid and economic tools for species recognition are needed. Our aim is to establish rolling circle amplification (RCA) as a screening tool for species-specific identification of Pseudallescheria and Scedosporium . With this aim, a set of padlock probes was designed on the basis of the internal transcribed spacer (ITS) region, differing by up to 13 fixed mutations. Padlock probes were unique as judged from sequence comparison by BLAST search in GenBank and in dedicated research databases at CBS (Centraalbureau voor Schimmelcultures Fungal Biodiversity Centre). RCA was applied as an in vitro tool, tested with pure DNA amplified from cultures. The species-specific padlock probes designed in this study yielded 100% specificity. The method presented here was found to be an attractive alternative to identification by restriction fragment length polymorphism (RFLP) or sequencing. The rapidity (
- Published
- 2011
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.