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Start Over Descriptor "Molybdenum -- Chemical properties" Publisher american society for microbiology
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1. Site-directed mutagenesis of the Anabaena sp. strain PCC 7120 nitrogenase active site to increase photobiological hydrogen production

Author

Masukawa, Hajime, Inoue, Kazuhito, Sakurai, Hidehiro, Wolk, C. Peter, and Hausinger, Robert P.

Subjects
Cyanobacteria -- Genetic aspects, Cyanobacteria -- Physiological aspects, Gene mutations -- Analysis, Hydrogen -- Chemical properties, Hydrogen -- Optical properties, Iron -- Chemical properties, Molybdenum -- Chemical properties, Nitrogenase -- Chemical properties, Biological sciences
Abstract

Hydrogen production by Anabaena sp. strain 7120 is increased by mutating six nitrogenase amino acid residues predicted to reside within the iron-molybdenum cofactor (FeMo-co) in order to direct electron flow selectively toward proton reduction in the presence of [N.sub.2]. The results have shown the feasibility of engineering cyanobacterial strain for enhanced photobiological production of [H.sub.2] in an aerobic, nitrogen-containing environment.

Published
2010

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